CSF1R antibody | ROS8G11
Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010).
CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, it has been shown to recognize canine CD115.
CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, it has been shown to recognize canine CD115.
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Horse Sheep Bovine Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant pCSF1R-Ig / Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Ba/F3 myeloma cell line.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CSF1R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| ELISA | |||
| Flow Cytometry 1 | 1/25 | 1/200 | |
| Immunofluorescence | |||
| Western Blotting |
- 1 Results may be enhanced using permeabilisation. Bio-Rad recommends the use of LeucopermTM (product code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |
|
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| Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | STAR117D405GA | F IF | 0.1 mg |
|
|
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg |
|
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| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
|
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| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
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| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:APC | MCA929APC | F | 100 Tests |
|
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| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
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| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
Source Reference
-
Moffatt, L. et al. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).
Dev Comp Immunol. 47: 123-8.
References for CSF1R antibody
-
Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
Dev Comp Immunol. 81: 95-104.
Further Reading
-
Kapetanovic, R. et al. (2013) The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.
BMC Genomics 14: 581. -
Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
J Leukoc Biol. 87: 753-64. -
Gow, D.J. et al. (2013) Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
Cytokine. 61 (2): 630-8.
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