CSF1R antibody | ROS8G11

Mouse anti Pig CSF1R

Product Type
Monoclonal Antibody
Clone
ROS8G11
Isotype
IgG2a
Specificity
CSF1R

Product Code Applications Pack Size List Price Your Price Qty
MCA5957GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * IF WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010).

CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, it has been shown to recognize canine CD115.

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Horse
Sheep
Bovine
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant pCSF1R-Ig / Fc fusion protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Ba/F3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/25 1/200
Immunofluorescence
Western Blotting
  1. 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Mouse IgG2a Negative Control

Source Reference

  1. Moffatt, L. et al. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).
    Dev Comp Immunol. 47: 123-8.

References for CSF1R antibody

  1. Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
    Dev Comp Immunol. 81: 95-104.

Further Reading

  1. Kapetanovic, R. et al. (2013) The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.
    BMC Genomics 14: 581.
  2. Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
    J Leukoc Biol. 87: 753-64.
  3. Gow, D.J. et al. (2013) Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
    Cytokine. 61 (2): 630-8.

Synonyms
CD115
CSF-1R
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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