CD5 antibody | 1H6/8
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F ResetMouse anti Pig CD5:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 1H6/8
- Isotype
- IgG2a
- Specificity
- CD5
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD5 antibody, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998).
Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- ConA/PMA activated porcine peripheral blood cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control:FITC | ||||||
References for CD5 antibody
-
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60 (3-4): 269-73. -
Saalmüller, A. et al. (1998) Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens.
Vet Immunol Immunopathol. 60 (3-4): 207-28. -
Doménech, N. et al. (2003) A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
Hybrid Hybridomics. 22 (3): 179-82. -
Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366. -
Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647. -
Jarosz, Ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
MCA2307F
150616
162881
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