CD31 antibody | LCI-9

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MCA1747
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Mouse anti Pig CD31 antibody, clone LCI-9 recognizes porcine Platelet endothelial cell adhesion molecule, also known as CD31 or PECAM-1. Porcine CD31 is a 740 amino acid ~140 kDa single pass type 1 transmembrane glycoprotein bearing six Ig-like C2-type domains. CD31 is expressed by various cell types, but particularly by endothelial cells where it is required for leukocyte transendothelial migration (UniProt:Q95242).

Mouse anti Pig CD31 antibody, clone LCI-9 has been used successfully for recognition of porcine CD31 by flow cytometry (Peterson et al. 2005) and immunofluorescence microscopy (Nasu et al. 1999).

Target Species
Pig
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Porcine CD31/human IgGFc fusion protein.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD31 antibody

  1. Peterson, M.D. et al. (2005) Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
    J Immunol. 174 (12): 8072-81.
  2. Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
    Transplantation. 1999 Sep 27;68(6): 861-7.
  3. Peterson, M.D. et al. (2005) Monocyte-induced endothelial calcium signaling mediates early xenogeneic endothelial activation.
    Am J Transplant. 5: 237-47.
  4. Harrower, T.P. et al. (2006) Long-term survival and integration of porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
    Exp Neurol. 197: 56-69.
  5. Al-Shalmani, S. et al. (2011) Quercetin and its principal metabolites, but not myricetin, oppose lipopolysaccharide-induced hyporesponsiveness of the porcine isolated coronary artery.
    Br J Pharmacol. 162: 1485-97.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunohistology - Frozen

Synonyms
PECAM-1
RRID
AB_2236793
UniProt
Q95242
Entrez Gene
PECAM1
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0030054 cell junction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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