CD205 antibody | ZH9F7
Mouse anti Pig CD205:FITC
- Product Type
- Monoclonal Antibody
- Clone
- ZH9F7
- Isotype
- IgG1
- Specificity
- CD205
Antibody Quality and Performance Guarantee
Figure B. FITC conjugated Mouse anti Pig CD14 antibody, clone MIL2 (MCA1218F) and Mouse anti Pig CD205 antibody, clone ZH9F7 (MCA6112GA) detected with RPE conjugated Goat anti Mouse IgG1 antibody (STAR132PE).
All experiments performed on frozen porcine cell lysed blood gated on live single cell monocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Figure B. FITC conjugated Mouse anti Pig CD14 antibody, clone MIL2 (MCA1218F) and Alexa Fluor®647 conjugated Mouse anti Pig CD205 antibody, clone ZH9F7 (MCA6112A647).
All experiments performed on frozen porcine cell lysed blood gated on live single cell monocytes, in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor®647 conjugated Mouse anti Pig CD14 antibody, clone MIL2 (MCA1218A647) and FITC conjugated Mouse anti Pig CD205 antibody, clone ZH9F7 (MCA6112F).
All experiments performed on porcine cell lysed blood gated on live single cell monocytes, in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Filter by Application:
F Reset| Mouse anti Pig CD205, clone ZH9F7 recognizes the endocytic receptor CD205, also known as DEC205. CD205 is expressed at high levels by dendritic cell (DC) subsets and can be detected on thymic epithelial cells ( Mouse anti Pig CD205 antibody, clone ZH9F7, has been used in characterization of the species-conserved features of the cDC1 subset. This subset is characterized by high surface expression of CD205, CD135, CADM1, low levels of CD172a, a lack of CD115, XCR1, and BATF3; and restricted APN gene expression (Auray et al. 2016). CD205 receptor expression was confirmed in cDC1 and cDC2 subsets using flow cytometry on porcine tonsil, submaxillary and mesenteric lymph nodes, and spleen lymphoid tissues ( |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Pig CD205
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD205 antibody
-
Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
J Immunol. 197 (12): 4791-806. -
Parra-Sánchez, H. et al. (2018) Characterization and expression of DEC205 in the cDC1 and cDC2 subsets of porcine dendritic cells from spleen, tonsil, and submaxillary and mesenteric lymph nodes.
Mol Immunol. 96: 1-7. -
Bustamante-Córdova, L. et al. (2019) Evaluation of a Recombinant Mouse X Pig Chimeric Anti-Porcine DEC205 Antibody Fused with Structural and Nonstructural Peptides of PRRS Virus.
Vaccines (Basel). 7 (2): 43. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16) :2103.
Further Reading
-
Lilian, F. et al. (2010) Characterization of porcine CD205
Developmental & Comparative Immunology. 34 (7): 715-721.
MCA6112F
151014
152497
164946
166603
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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