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Obinutuzumab antibody | AbD51167ad

Human anti Obinutuzumab

Product Type
Monoclonal Antibody
Clone
AbD51167ad
Isotype
Fab antibody
Specificity
Obinutuzumab

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New TZA048
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Obinutuzumab Antibody, clone AbD51167ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug obinutuzumab, and its biosimilar products. It does not recognize the drug target, the B cell surface marker CD20, nor obinutuzumab in complex with CD20. This antibody can be used to measure free obinutuzumab levels in serum from patients.

A pair of anti-obinutuzumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD51167ad is recommended as a capture antibody, paired with Human Anti-Obinutuzumab Antibody, clone AbD51296 (TZA049) as the detection antibody.

TZA048 a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog.

Obinutuzumab (trade name Gazyvaro) is a humanized monoclonal antibody of the IgG1/kappa isotype that specifically targets the extracellular loop of CD20 when expressed at the surface of non malignant and malignant pre-B and mature B-cells. It does not bind CD20 expressed on hematopoietic stem cells, pro-B cells, normal plasma cells or other normal tissue. In non-clinical studies, obinutuzumab induces direct cell death and mediates antibody dependent cellular cytotoxicity (ADCC) and antibody dependent cellular phagocytosis (ADCP) through recruitment of FcɣRIII positive immune effector cells. In vivo, obinutuzumab mediates a low degree of complement dependent cytotoxicity (CDC). Obinutuzumab is indicated for the combination treatment of patients with chronic lymphocytic leukemia and follicular lymphoma.

View a summary of all anti-obinutuzumab antibodies.

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from Bio-Rad’s human antibody phage display libraries expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Obinutuzumab
Affinity
The monovalent intrinsic affinity of Human anti-Obinutuzumab Antibody, clone AbD51167ad was measured as KD< = 0.096 nM by real time, label-free molecular interaction analysis on immobilized obinutuzumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Gazyvaro is a registered trademark of Genentech/ Roche and Biogen.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD51167ad may be used as the capture antibody together with AbD51296ad (TZA049) or AbD51296pap (TZA049P) as the detection reagent for obinutuzumab in a PK bridging ELISA.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Obinutuzumab HCA403 E 0.1 mg loader
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loader
Description Human anti Obinutuzumab
Human anti Obinutuzumab HCA404 E 0.1 mg loader
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loader
Description Human anti Obinutuzumab
Human anti Obinutuzumab HCA405 E 0.1 mg loader
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Description Human anti Obinutuzumab
Human anti Obinutuzumab TZA049 E 0.1 mg
List Price Your Price
Description Human anti Obinutuzumab
Human anti Obinutuzumab:HRP TZA049P E 0.1 mg
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Description Human anti Obinutuzumab:HRP
BiSpyCatcher2:HRP TZC002P IY 1 mg
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Description BiSpyCatcher2:HRP

Synonyms
Gazyvaro
Licensed Use
For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

TZA048

167085

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