Obinutuzumab antibody | AbD51296
Human anti Obinutuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD51296
- Isotype
- Fab antibody
- Specificity
- Obinutuzumab
Human Anti-Obinutuzumab Antibody, clone AbD51296 is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug obinutuzumab, and its biosimilar products. It does not recognize the drug target, the B cell surface marker CD20, nor obinutuzumab in complex with CD20. This antibody can be used to measure free obinutuzumab levels in serum from patients. Clone AbD51296 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA049. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA049P. A pair of anti-obinutuzumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD51296 is recommended as a detection antibody, paired with Human Anti-Obinutuzumab Antibody, clone AbD51167ad (TZA048) as the capture reagent. Obinutuzumab (trade name Gazyvaro) is a humanized monoclonal antibody of the IgG1/kappa isotype that specifically targets the extracellular loop of CD20 when expressed at the surface of non malignant and malignant pre-B and mature B-cells. It does not bind CD20 expressed on hematopoietic stem cells, pro-B cells, normal plasma cells or other normal tissue. In non-clinical studies, obinutuzumab induces direct cell death and mediates antibody dependent cellular cytotoxicity (ADCC) and antibody dependent cellular phagocytosis (ADCP) through recruitment of FcɣRIII positive immune effector cells. In vivo, obinutuzumab mediates a low degree of complement dependent cytotoxicity (CDC). Obinutuzumab is indicated for the combination treatment of patients with chronic lymphocytic leukemia and follicular lymphoma. View a summary of all anti-obinutuzumab antibodies. |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from Bio-Rad’s human antibody phage display libraries expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Obinutuzumab
- Affinity
- The monovalent intrinsic affinity of Human anti-Obinutuzumab Antibody, clone AbD51296ad was measured as KD = 0.293 nM by real time, label-free molecular interaction analysis on immobilized obinutuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Gazyvaro is a registered trademark of Genentech/ Roche and Biogen. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD51296ad may be used as the detection antibody together with AbD51167ad (TZA048) as the capture antibody for obinutuzumab in a PK bridging ELISA.
Protocol: PK bridging ELISA
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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SpyTag3 Peptide | BLP086 | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | SpyTag3 Peptide | ||||||
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Obinutuzumab | HCA403 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Obinutuzumab | ||||||
Human anti Obinutuzumab | HCA404 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Obinutuzumab | ||||||
Human anti Obinutuzumab | HCA405 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Obinutuzumab | ||||||
Human anti Obinutuzumab | TZA048 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Obinutuzumab | ||||||
Human anti Obinutuzumab:HRP | TZA049P | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Obinutuzumab:HRP | ||||||
BiSpyCatcher2:HRP | TZC002P | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | BiSpyCatcher2:HRP |
- Synonyms
- Gazyvaro
TZA049
167086If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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