Nirsevimab antibody | AbD64623ad
Human anti Nirsevimab
- Product Type
- Monoclonal Antibody
- Clone
- AbD64623ad
- Isotype
- Fab antibody
- Specificity
- Nirsevimab
Antibody Quality and Performance Guarantee
TZA0147
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Nirsevimab Antibody, clone AbD64623ad (TZA0147) at a concentration of 2 μg/mL in PBST followed by an HRP labelled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with Nirsevimab, human IgG1 kappa control, human VH/VL subclass control, and normal human serum at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Nirsevimab Antibody, clone AbD64623ad (TZA0147) titrated to the given concentrations in PBST followed by an HRP conjugated Anti-DYKDDDDK tag Antibody in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for nirsevimab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Nirsevimab Antibody, clone AbD64623ad (TZA0147) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of nirsevimab. Detection was performed using HRP conjugated Human Anti-Nirsevimab Antibody, clone AbD64624pap (TZA0148P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Fusion glycoprotein F0 of RSV at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of Nirsevimab (0.15 µg/ml) plus increasing concentrations of Human Anti-Nirsevimab Antibody, clone AbD64623ad (TZA0147) was added. Free nirsevimab, still capable of binding to the Fusion glycoprotein F0 of RSV coated plate was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
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E Reset| Human Anti-Nirsevimab Antibody, clone AbD64623ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug nirsevimab, and its biosimilar products. It does not recognize the free Fusion glycoprotein F0 of RSV, nor nirsevimab in complex with Fusion glycoprotein F0 of RSV. This antibody can be used to measure free nirsevimab levels in serum from patients. A pair of anti-nirsevimab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD64623ad is recommended as a capture antibody, paired with Human Anti-Nirsevimab Antibody, clone AbD64624pap (TZA0148P) as the detection antibody. TZA0147 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Nirsevimab (trade name Beyfortus) is a humanized monoclonal antibody of the IgG1/kappa isotype that targets the prefusion conformation of the RSV F protein, a glycoprotein involved in the membrane fusion step of the viral entry process and neutralises several RSV A and B strains. Nirsevimab is indicated to treat respiratory syncytial virus (RSV) lower respiratory tract disease in neonates and infants. View a summary of all anti-nirsevimab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Nirsevimab
- Affinity
- The monovalent intrinsic affinity of AbD64623ad was measured as KD = 0.1 nM by real time, label free molecular interaction analysis on immobilized nirsevimab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Beyfortus is a trademark of Sanofi and AstraZeneca. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD64623ad can be used in indirect ELISA to detect nirsevimab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for nirsevimab together with TZA0148 or TZA0148P as the detection reagent.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Nirsevimab | HCA450 | E | 0.1 mg |
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| Description | Human anti Nirsevimab | ||||||
| Human anti Nirsevimab | HCA451 | E | 0.1 mg |
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| Description | Human anti Nirsevimab | ||||||
| Human anti Nirsevimab | HCA452 | E | 0.1 mg |
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| Description | Human anti Nirsevimab | ||||||
| Human anti Nirsevimab | TZA0148 | E | 0.1 mg |
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| Description | Human anti Nirsevimab | ||||||
| Human anti Nirsevimab:HRP | TZA0148P | E | 0.1 mg |
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| Description | Human anti Nirsevimab:HRP | ||||||
- Synonyms
- Beyfortus
TZA0147
172491
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Nirsevimab specificity
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