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Ly-6G antibody | 1A8

Rat anti Mouse Ly-6G:FITC

Product Type
Monoclonal Antibody

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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Rat anti Mouse Ly-6G antibody, clone 1A8 recognizes lymphocyte antigen 6 complex, locus G (Ly-6G).

Ly-6G is a cell surface, glycosylphosphatidylinositol (GPI) anchored protein, expressed on granulocytes in the bone marrow and peripheral blood. Clone 1A8 has been used in vivo in mice to deplete neutrophils (Daley et al. 2008).

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Ly-6G transfected EL-4J cell line
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized LEW/N rats were fused with cells from the murine SP2/0 myeloma.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
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Description Rat IgG2a Negative Control:FITC

Source Reference

  1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
    J Immunol. 151 (5): 2399-408.

References for Ly-6G antibody

  1. Gabrielli, E. et al. (2018) Saccharomyces cerevisiae-based probiotic as novel anti-fungal and anti-inflammatory agent for therapy of vaginal candidiasis.
    Benef Microbes. 9 (2): 219-30.
  2. Rabadi, M.M. et al. (2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator).
    Am J Physiol Renal Physiol. Apr 03 [Epub ahead of print].

Entrez Gene
GO Terms
GO:0009897 external side of plasma membrane
GO:0031225 anchored to membrane


157883 162551 180809

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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