Ly-6G antibody | 1A8
Rat anti Mouse Ly-6G:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 1A8
- Isotype
- IgG2a
- Specificity
- Ly-6G
Antibody Quality and Performance Guarantee
| Rat anti Mouse Ly-6G antibody, clone 1A8 recognizes lymphocyte antigen 6 complex, locus G (Ly-6G). Ly-6G is a cell surface, glycosylphosphatidylinositol (GPI) anchored protein, expressed on granulocytes in the bone marrow and peripheral blood. Clone 1A8 has been used in vivo in mice to deplete neutrophils (Daley et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Ly-6G transfected EL-4J cell line
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized LEW/N rats were fused with cells from the murine SP2/0 myeloma.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control:FITC | ||||||
Source Reference
-
Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
J Immunol. 151 (5): 2399-408.
References for Ly-6G antibody
-
Gabrielli, E. et al. (2018) Saccharomyces cerevisiae-based probiotic as novel anti-fungal and anti-inflammatory agent for therapy of vaginal candidiasis.
Benef Microbes. 9 (2): 219-30. -
Rabadi, M.M. et al. (2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO.
Am J Physiol Renal Physiol. 316 (6): F1180-F1190. -
Zhao, Y. et al. (2024) Costunolide Inhibits Chronic Kidney Disease Development by Attenuating IKKβ/NF-κB Pathway
Drug Design, Development and Therapy. Volume 18: 2693-712.
- Synonyms
- Gr-1
- UniProt
- P35461
- Entrez Gene
- Ly6g
- GO Terms
- GO:0009897 external side of plasma membrane
- GO:0031225 anchored to membrane
MCA6077F
157883
162551
180809
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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