Ly-6C antibody | ER-MP20
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|Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.
Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1 ml distilled water
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Balb/c macrophage precursor cell hybrids.
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced through the use of the SeroBlock FcR reagent.
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References for Ly-6C antibody
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Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34.
de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
J Immunol Methods. 217 (1-2): 27-39.
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Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15: 515-24.
Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158 (1): 131-45.
View The Latest Product References
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Cancer Res. 72: 876-86.
Chan, J. et al. (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
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van Rijt, L.S. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
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J Nutr. 142: 803-8.
Henkel, G. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.
Bossaller, L. et al. (2013) Overexpression of membrane-bound fas ligand (CD95L) exacerbates autoimmune disease and renal pathology in pristane-induced lupus.
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Mooney, J.E. et al. (2010) Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response.
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Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
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Movahedi, K. et al. (2012) Nanobody-based targeting of the macrophage mannose receptor for effective in vivo imaging of tumor-associated macrophages.
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Ribechini, E. et al. (2009) Gr-1 antibody induces STAT signaling, macrophage marker expression and abrogation of myeloid-derived suppressor cell activity in BM cells.
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Bossaller, L. et al. (2016) TLR9 Deficiency Leads to Accelerated Renal Disease and Myeloid Lineage Abnormalities in Pristane-Induced Murine Lupus.
J Immunol. 197 (4): 1044-53.
Barnes, M.A. et al. (2015) Macrophage migration inhibitory factor is required for recruitment of scar-associated macrophages during liver fibrosis.
J Leukoc Biol. 97 (1): 161-9.
Ohnishi, K. et al. (2012) Immunohistochemical detection of possible cellular origin of hepatic histiocytic sarcoma in mice.
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Van den Bossche. J. et al. (2012) Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
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Greifenberg, V. et al. (2009) Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development.
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Cardona, S.M.et al. (2015) Disruption of Fractalkine Signaling Leads to Microglial Activation and Neuronal Damage in the Diabetic Retina.
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Robbie, S.J. et al. (2016) Enhanced Ccl2-Ccr2 signaling drives more severe choroidal neovascularization with aging.
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