Ly-6C antibody | ER-MP20

Rat anti Mouse Ly-6C:RPE

Product Type
Monoclonal Antibody
Clone
ER-MP20
Isotype
IgG2a
Specificity
Ly-6C

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MCA2389PE
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Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.

Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).

Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Balb/c macrophage precursor cell hybrids.
Fusion Partners
Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced through the use of the SeroBlock FcR reagent.

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Description Rat IgG2a Negative Control:RPE

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Description Mouse Seroblock FcR
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References for Ly-6C antibody

  1. Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model of Yersinia pseudotuberculosis infection.
    Infect Immun 78: 2329-41.
  2. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20 (1): 27-34.
  3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
    J Immunol Methods. 217 (1-2): 27-39.
  4. Schatteman, G.C. et al. (2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.
    Am J Pathol. 177: 2002-10.
  5. Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
    J Invest Dermatol. 121: 280-8.
  6. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
    Mol Ther. 17: 65-72.
  7. Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int Immunol. 15: 515-24.
  8. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
    Am J Pathol. 158 (1): 131-45.
  9. View The Latest Product References
  10. Lesokhin, A.M. et al. (2012) Monocytic CCR2+ Myeloid-Derived Suppressor Cells Promote Immune Escape by Limiting Activated CD8 T-cell Infiltration into the Tumor Microenvironment.
    Cancer Res. 72: 876-86.
  11. Chan, J. et al. (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
    Blood. 92: 1423-31.
  12. van Rijt, L.S. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
    Blood. 100: 3663-71.
  13. Arnardottir, H.H.et al. (2012) Dietary Fish Oil Decreases the Proportion of Classical Monocytes in Blood in Healthy Mice but Increases Their Proportion upon Induction of Inflammation.
    J Nutr. 142: 803-8.
  14. Henkel, G. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.
    Blood. 93:2849-58.
  15. Bossaller, L. et al. (2013) Overexpression of membrane-bound fas ligand (CD95L) exacerbates autoimmune disease and renal pathology in pristane-induced lupus.
    J Immunol. 191: 2104-14.
  16. Garcia, J.A. et al. (2013) Regulation of adaptive immunity by the fractalkine receptor during autoimmune inflammation.
    J Immunol. 191: 1063-72.
  17. Benoit, S. et al. (2015) Murine Liver Myeloid Cell Isolation Protocol
    BIO-PROTOCOL. 5 (10) [Epub ahead of print].
  18. Damya, L. et al. (2014) Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
    BIO-PROTOCOL. 4 (22) [Epub ahead of print].
  19. Morganti, J.M. et al. (2016) Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.
    J Neuroinflammation. 13 (1): 80.
  20. Mooney, J.E. et al. (2010) Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response.
    Am J Pathol. 176 (1): 369-80.
  21. Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
    Cell Tissue Res. 346 (1): 99-109.
  22. Movahedi, K. et al. (2012) Nanobody-based targeting of the macrophage mannose receptor for effective in vivo imaging of tumor-associated macrophages.
    Cancer Res. 72 (16): 4165-77.
  23. Ribechini, E. et al. (2009) Gr-1 antibody induces STAT signaling, macrophage marker expression and abrogation of myeloid-derived suppressor cell activity in BM cells.
    Eur J Immunol. 39 (12): 3538-51.
  24. Bossaller, L. et al. (2016) TLR9 Deficiency Leads to Accelerated Renal Disease and Myeloid Lineage Abnormalities in Pristane-Induced Murine Lupus.
    J Immunol. 197 (4): 1044-53.
  25. Barnes, M.A. et al. (2015) Macrophage migration inhibitory factor is required for recruitment of scar-associated macrophages during liver fibrosis.
    J Leukoc Biol. 97 (1): 161-9.
  26. Ohnishi, K. et al. (2012) Immunohistochemical detection of possible cellular origin of hepatic histiocytic sarcoma in mice.
    J Clin Exp Hematop. 52 (3): 171-7.
  27. Van den Bossche. J. et al. (2012) Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
    Scand J Immunol. 75 (6): 588-98.
  28. Houthuys, E. et al. (2010) A method for the isolation and purification of mouse peripheral blood monocytes.
    J Immunol Methods. 359 (1-2): 1-10.
  29. Greifenberg, V. et al. (2009) Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development.
    Eur J Immunol. 39 (10): 2865-76.
  30. Cardona, S.M.et al. (2015) Disruption of Fractalkine Signaling Leads to Microglial Activation and Neuronal Damage in the Diabetic Retina.
    ASN Neuro. 7 (5)Oct 29 [Epub ahead of print].
  31. Waddell, A. et al. (2011) Colonic eosinophilic inflammation in experimental colitis is mediated by Ly6C(high) CCR2(+) inflammatory monocyte/macrophage-derived CCL11.
    J Immunol. 186 (10): 5993-6003.
  32. Robbie, S.J. et al. (2016) Enhanced Ccl2-Ccr2 signaling drives more severe choroidal neovascularization with aging.
    Neurobiol Aging. 40: 110-9.
  33. Cao, Y. et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
    J Leukoc Biol. 100 (3): 513-23.
  34. Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
    J Cell Physiol. 232 (12): 3273-85.
  35. Khedoe, P.P.S.J. et al. (2017) Acute and chronic effects of treatment with mesenchymal stromal cells on LPS-induced pulmonary inflammation, emphysema and atherosclerosis development.
    PLoS One. 12 (9): e0183741.
  36. Koohy, H. et al. (2018) Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells.
    Genome Biol. 19 (1): 126.
  37. Pluijmert, N.J. et al. (2020) Effects on cardiac function, remodeling and inflammation following myocardial ischemia-reperfusion injury or unreperfused myocardial infarction in hypercholesterolemic APOE*3-Leiden mice.
    Sci Rep. 10 (1): 16601.
  38. Ascone, G. et al. (2020) Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
    Int J Mol Sci. 21 (11): 3774.
  39. Pluijmert, N.J. et al. (2021) Phosphorylcholine antibodies restrict infarct size and left ventricular remodelling by attenuating the unreperfused post-ischaemic inflammatory response.
    J Cell Mol Med. 25 (16): 7772-82.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Synonyms
Lymphocyte Antigen 6C2
RRID
AB_2234744
UniProt
P0CW03
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