Kappa Light Chain antibody | LO-MK-1
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Rat anti Mouse kappa light chain antibody, clone LO-MK-1 recognises the murine kappa immunoglobulin light chain, and does not cross-react murine immunoglobulin heavy chains or lambda light chains.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Thiomersal 50% Glycerol - Immunogen
- Purified mouse IgG1 kappa.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01837
- Entrez Gene
- Igk-C
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of Kappa Light Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 500ng/ml | 1000ng/ml | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with MCA1589 as the capture reagent.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Kappa Light Chain antibody
-
Delaunay, T. et al. (1990) Rat (and mouse) monoclonal antibodies. VIII. ELISA measurement of Ig production in mouse hybridoma culture supernatants.
J Immunol Methods. 131 (1): 33-9. -
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
J Exp Med. pii: jem.20161807. [Epub ahead of print]
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