Kappa Light Chain antibody | LO-MK-1

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Rat anti Mouse Kappa Light Chain:HRP

Product Type
Monoclonal Antibody
Clone
LO-MK-1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1291P C E 0.5 mg
Rat anti Mouse kappa light chain antibody, clone LO-MK-1 recognises the murine kappa immunoglobulin light chain, and does not cross-react murine immunoglobulin heavy chains or lambda light chains.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Immunogen
Purified mouse IgG1 kappa.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01837 Related reagents
Entrez Gene
Igk-C Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of Kappa Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml 1000ng/ml
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with MCA1589 as the capture reagent.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Kappa Light Chain antibody

  1. Delaunay, T. et al. (1990) Rat (and mouse) monoclonal antibodies. VIII. ELISA measurement of Ig production in mouse hybridoma culture supernatants.
    J Immunol Methods. 131 (1): 33-9.
  2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  3. Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
    J Exp Med. pii: jem.20161807. [Epub ahead of print]