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JAM-C antibody | CRAM-19 H36

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Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 1 Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 2 Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 3
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 1
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:FITC (MCA5934F)
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 2
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C (MCA5934) followed by Rabbit F(ab')2 anti Rat IgG:FITC (STAR17B)
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 3
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:Biotin (MCA5934B) followed by Streptavidin:FITC (STAR2B).

Rat anti Mouse JAM-C:Biotin

Product Type
Monoclonal Antibody
Clone
CRAM-19 H36
Isotype
IgG2a
Specificity
JAM-C

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Product Code Applications Pack Size List Price Your Price Qty
MCA5934B
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C E F IF IP 0.1 mg loader
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Rat anti Mouse JAM-C, clone CRAM-19 H36 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.

JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant soluble JAM-C.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Fischer rats were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry Neat 1/5
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for JAM-C antibody

  1. Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
    J Biol Chem. 276 (4): 2733-41.
  2. Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
    Blood. 100 (7): 2479-86.
  3. Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
    J Exp Med. 207 (6): 1173-82.

Flow Cytometry

Immunoprecipitation

Synonyms
JAM-3
UniProt
Q9D8B7
Q9BX67
Entrez Gene
JAM3
Jam3
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0050900 leukocyte migration
GO:0001780 neutrophil homeostasis
GO:0002250 adaptive immune response
GO:0002318 myeloid progenitor cell differentiation
GO:0002523 leukocyte migration involved in inflammatory response
GO:0005178 integrin binding
GO:0005923 tight junction
GO:0030010 establishment of cell polarity
GO:0007160 cell-matrix adhesion
GO:0007286 spermatid development
GO:0033010 paranodal junction
GO:0042552 myelination
GO:0042803 protein homodimerization activity
GO:0046982 protein heterodimerization activity
GO:0090138 regulation of actin cytoskeleton organization by cell-cell adhesion

MCA5934B

1112 162413

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