JAM-C antibody | CRAM-19 H36
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F ResetRat anti Mouse JAM-C
- Product Type
- Monoclonal Antibody
- Clone
- CRAM-19 H36
- Isotype
- IgG2a
- Specificity
- JAM-C
Quick Links:
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| Rat anti Mouse JAM-C, clone CRAM-19 H36 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2. JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant soluble JAM-C.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for JAM-C antibody
-
Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
J Biol Chem. 276 (4): 2733-41. -
Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
Blood. 100 (7): 2479-86. -
Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
J Exp Med. 207 (6): 1173-82.
- Synonyms
- JAM-3
- UniProt
- Q9D8B7
- Q9BX67
- Entrez Gene
- JAM3
- Jam3
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0050900 leukocyte migration
- GO:0001780 neutrophil homeostasis
- GO:0002250 adaptive immune response
- GO:0002318 myeloid progenitor cell differentiation
- GO:0002523 leukocyte migration involved in inflammatory response
- GO:0005178 integrin binding
- View More GO Terms
- GO:0005923 tight junction
- GO:0030010 establishment of cell polarity
- GO:0007160 cell-matrix adhesion
- GO:0007286 spermatid development
- GO:0033010 paranodal junction
- GO:0042552 myelination
- GO:0042803 protein homodimerization activity
- GO:0046982 protein heterodimerization activity
- GO:0090138 regulation of actin cytoskeleton organization by cell-cell adhesion
View more products with JAM-C specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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