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JAM-C antibody | CRAM-19 H36

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Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 1 Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 2 Anti Mouse JAM-C Antibody, clone CRAM-19 H36 thumbnail image 3
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 1
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:FITC (MCA5934F)
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 2
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C (MCA5934) followed by Rabbit F(ab')2 anti Rat IgG:FITC (STAR17B)
Anti Mouse JAM-C Antibody, clone CRAM-19 H36 gallery image 3
Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:Biotin (MCA5934B) followed by Streptavidin:FITC (STAR2B).

Rat anti Mouse JAM-C:Biotin

Rat anti Mouse JAM-C:FITC

Rat anti Mouse JAM-C

Product Type
Monoclonal Antibody
Clone
CRAM-19 H36
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA5934B
Datasheet
C F IF IP
SDS
0.1 mg loader

MCA5934F
Datasheet
F IF
SDS
0.1 mg loader

MCA5934
Datasheet
C F IF IP
SDS
0.25 mg loader

Rat anti Mouse JAM-C, clone CRAM-19 H36 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.

JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant soluble JAM-C.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q9D8B7
Q9BX67
Entrez Gene
JAM3
Jam3
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0050900 leukocyte migration
GO:0001780 neutrophil homeostasis
GO:0002250 adaptive immune response
GO:0002318 myeloid progenitor cell differentiation
GO:0002523 leukocyte migration involved in inflammatory response
GO:0005178 integrin binding
GO:0005923 tight junction
GO:0030010 establishment of cell polarity
GO:0007160 cell-matrix adhesion
GO:0007286 spermatid development
GO:0033010 paranodal junction
GO:0042552 myelination
GO:0042803 protein homodimerization activity
GO:0046982 protein heterodimerization activity
GO:0090138 regulation of actin cytoskeleton organization by cell-cell adhesion
Regulatory
For research purposes only

Applications of JAM-C antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat 1/10
Immunofluorescence
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Flow Cytometry

Immunoprecipitation

Product Specific References

References for JAM-C antibody

  1. Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
    J Biol Chem. 276 (4): 2733-41.
  2. Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
    Blood. 100 (7): 2479-86.
  3. Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
    J Exp Med. 207 (6): 1173-82.

Fluorescent Spectraviewer

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