IgG1 antibody | AbD24121
25 μg of HeLa cell lysate spiked with a primary antibody mixture of Mouse IgG1 anti actin beta (VMA00048), Mouse IgG2a anti PCNA (VMA00018) and Mouse IgG2b anti ezrin (VMA00344) each at 0.5 μg/ml were run in lanes 2, 4, 6, 8 and 10 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to PVDF membrane. Bio-Rad Precision Plus MW marker was run in lanes 1, 3, 5, 7, 9 and 11.
Mouse anti actin beta (VMA00048) 42 kDa, was detected using Human anti Mouse IgG1 conjugated to DyLight488 (clone AbD24121, HCA309D488) in lane 4. Clone AbD24121 does not recognize Mouse anti PCNA IgG2a or Mouse anti ezrin IgG2b.
Mouse anti PCNA (VMA00018) 29 kDa, was detected using Human anti Mouse IgG2a conjugated to DyLight650 (clone AbD24124, HCA310D650) in lane 6. Clone AbD24124 does not recognize Mouse anti actin beta IgG1 or Mouse anti ezrin IgG2b.
Mouse anti ezrin (VMA00344) 80 kDa, was detected using Human anti Mouse IgG2b conjugated to DyLight550 (clone AbD24127, HCA311D550) in lane 8. Clone AbD24127 does not recognize Mouse anti actin beta IgG1 or Mouse anti PCNA IgG2a.
Goat anti Mouse IgG antibody conjugated to Dylight549 (STAR117D549GA) was added to lane 10 as a standard antibody which detects all three isotypes.
All detection antibodies were used at 0.3 μg/ml. The blot was visualized using the Chemidoc™ imaging system
Human anti Mouse IgG1:Alk. Phos.
Human anti Mouse IgG1:HRP
Human anti Mouse IgG1:RPE
Human anti Mouse IgG1:DyLight®488
Human anti Mouse IgG1:DyLight®550
Human anti Mouse IgG1:DyLight®650
- Product Type
- Monoclonal Antibody
- Clone
- AbD24121
- Isotype
- HuCAL Fab bivalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA309A | E P | 0.1 mg |
|
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| HCA309P | E P | 0.1 mg |
|
|
| HCA309PE | F | 0.1 mg |
|
|
| HCA309D488 | F IF WB | 0.1 mg |
|
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| HCA309D550 | F IF WB | 0.1 mg |
|
|
| HCA309D650 | F IF WB | 0.1 mg |
|
Human anti Mouse IgG1 monoclonal antibody, clone AbD24121, recognizes the IgG1 isotype of the primary antibody, with no species cross reactivity against mouse IgG2a and mouse IgG2b primary antibodies. This antibody allows for more than one unlabelled mouse monoclonal antibody to be used at any one time permitting multiplexing.
Product Details
- Target Species
- Mouse
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to Alkaline Phosphatase - liquid.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight®488 - liquid
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight®550 - liquid
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight®650 - liquid
- Reconstitution
- Reconstitute with 100 ul distilled water
- Preparation
- Prepared by affinity chromatography
- Preparation
- Prepared by affinity chromatography
- Preparation
- Prepared by affinity chromatography
- Preparation
- Prepared by affinity chromatography
- Preparation
- Prepared by affinity chromatography
- Preparation
- Prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences - Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. - Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. - Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. - Regulatory
- For research purposes only
Applications of IgG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin | 1/100 | 1/1000 | |
| ELISA | |||
| Immunohistology - Paraffin | 1/100 | 1/1000 | |
| Flow Cytometry | 1/500 | 1/10000 | |
| Flow Cytometry | 1/500 | 1/10,000 | |
| Immunofluorescence | 1/100 | 1/1000 | |
| Western Blotting | 1/1000 | 1/10,000 | |
| Flow Cytometry | 1/500 | 1/10,000 | |
| Immunofluorescence | 1/100 | 1/1000 | |
| Western Blotting | 1/1000 | 1/10,000 | |
| Flow Cytometry | 1/500 | 1/10,000 | |
| Immunofluorescence | 1/100 | 1/1000 | |
| Western Blotting | 1/1000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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