CD86 antibody | PO3

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Anti Mouse CD86 Antibody, clone PO3 thumbnail image 1

Anti Mouse CD86 Antibody, clone PO3 gallery image 1 — Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC (MCA2463F)

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Rat anti Mouse CD86:FITC

Product Type: Monoclonal Antibody
Clone: PO3
Isotype: IgG2b
Specificity: CD86

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References (6)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2463F Datasheet Datasheet SDS SDS	F	0.1 mg		Log in	Get Quote
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Rat anti mouse CD86 antibody, clone PO3 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses.

Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86 (Nakajima et al. 1997).

Target Species: Mouse
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: Mouse B-cell line, BCL1.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Spleen cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

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References for CD86 antibody

Jin, L.P. et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
J Immunol. 173 (6): 3612-9.
Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
J Immunol. 167 (9): 4981-6.
Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
Int Immunol. 9 (5): 637-44.
Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
J Clin Invest. 119 (12): 3723-38.
Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
J Immunol. 187: 4517-29.
Lei, Y. et al. (2021) miR-129-5p Ameliorates Ischemic Brain Injury by Binding to SIAH1 and Activating the mTOR Signaling Pathway.
J Mol Neurosci. 71 (9): 1761-71.

Synonyms: B7-2
RRID: AB_808461
UniProt: P42082
Entrez Gene: Cd86
GO Terms: GO:0002224 toll-like receptor signaling pathway; GO:0016021 integral to membrane; GO:0004872 receptor activity; GO:0009897 external side of plasma membrane; GO:0042113 B cell activation; GO:0043231 intracellular membrane-bounded organelle