CD86 antibody | PO3
Rat anti mouse CD86 antibody, clone PO3 (MCA2463) used to lable M1 macrophages by immunohistochemistry on cryosections.
Image caption:
Quantification of different key markers of tumor infiltrating immune cells, as follows: CD86+ M1 and CD206+ M2 (panel A),
per HMMF in BALB/c mice injected with C51 P, C51 E, and C51 FL Rnaset2 cell lines (N = six animals in group). The mean ± SE are indicated. Immunohistochemistry analyses of tumor sections from BALB/c mice injected with C51 P, C51 E, and C51 FL Rnaset2 cell lines at indicated time points are shown. An ANOVA statistical analysis was performed assuming p *lt;0.05 as a threshold value. * p <0.05; ** p <0.01; *** p <0.001.
From: De Vito A, Orecchia P, Balza E, Reverberi D, Scaldaferri D, Taramelli R, Noonan DM, Acquati F, Mortara L.
Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection.
Cancers (Basel). 2020 Mar 18;12(3):717.
doi: 10.3390/cancers12030717.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD86:FITC
- Product Type
- Monoclonal Antibody
- Clone
- PO3
- Isotype
- IgG2b
- Specificity
- CD86
Antibody Quality and Performance Guarantee
| Rat anti mouse CD86 antibody, clone PO3 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86 (Nakajima et al. 1997). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Mouse B-cell line, BCL1.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
References for CD86 antibody
-
Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
Int Immunol. 9 (5): 637-44. -
Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
J Immunol. 167 (9): 4981-6. -
Jin, L.P. et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
J Immunol. 173 (6): 3612-9. -
Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
J Clin Invest. 119 (12): 3723-38. -
Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
J Immunol. 187: 4517-29. -
Lei, Y. et al. (2021) miR-129-5p Ameliorates Ischemic Brain Injury by Binding to SIAH1 and Activating the mTOR Signaling Pathway.
J Mol Neurosci. 71 (9): 1761-71. -
Li, T. et al. (2019) NLRP3 inflammasome-activating arginine-based liposomes promote antigen presentations in dendritic cells.
Int J Nanomedicine. 14: 3503-16. -
Chen, Z. et al. (2023) Vascularized polypeptide hydrogel modulates macrophage polarization for wound healing.
Acta Biomater. 155: 218-34.
View The Latest Product References
-
De Vito, A. et al. (2020) Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection.
Cancers (Basel). 12 (3)Mar 18 [Epub ahead of print]. -
Wang, C. et al. (2024) The Role of TLR7 and TLR9 in the Pathogenesis of Systemic Sclerosis.
Int J Mol Sci. 25 (11): 6133.
- Synonyms
- B7-2
- RRID
- AB_808461
- UniProt
- P42082
- Entrez Gene
- Cd86
- GO Terms
- GO:0002224 toll-like receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- GO:0042113 B cell activation
- GO:0043231 intracellular membrane-bounded organelle
MCA2463F
158542
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