CD83 antibody | 3D11
Rat anti Mouse CD83:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 3D11
- Isotype
- IgG2a
- Specificity
- CD83
Antibody Quality and Performance Guarantee
Filter by Application:
F Reset| Rat anti Mouse CD83 antibody, clone 3D11 recognizes murine CD83, a 45 kDa type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen presentation, thymic T-cell maturation, B-cell function and peripheral T-cell activation. Activated dendritic cells and B-cells release a soluble form of CD83 that is reported to have an immunosuppressive effect, suggesting a therapeutic role for sCD83 in the regulation of immune disorders. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized rats were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rat IgG2a Negative Control:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
References for CD83 antibody
-
El-Ashmawy, N.E. et al. (2015) In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells.
Journal of Genetic Engineering and Biotechnology. 13 (2): 185-92. -
He, Y.T. et al. (2016) In vitro generation of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.
Oncol Lett. 12 (2): 1101-6.
Further Reading
-
Fujimoto, Y. & Tedder, T.F. (2006) CD83: a regulatory molecule of the immune system with great potential for therapeutic application.
J Med Dent Sci. 53 (2): 85-91.
- Synonyms
- HB15
- RRID
- AB_2074744
- UniProt
- O88324
- Entrez Gene
- Cd83
- GO Terms
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
- GO:0014070 response to organic cyclic compound
- GO:0032713 negative regulation of interleukin-4 production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032743 positive regulation of interleukin-2 production
- GO:0043372 positive regulation of CD4-positive, alpha beta T cell differentiation
MCA2747F
153803
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD83 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up
