CD8 Beta antibody | YTS156.7.7

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Rat anti Mouse CD8 Beta:RPE

Product Type
Monoclonal Antibody
Clone
YTS156.7.7
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2805PE F 0.5 ml
Rat anti Mouse CD8 beta antibody, clone YTS156.7.7 recognizes the murine T-cell surface glycoprotein CD8 beta chain, also known as CD8β, LY-3 or Lyt-3. CD8β is a 213 amino acid ~25 kDa single pass type-1 transmembrane glycoprotein bearing a single Ig-like V-type domain (UniProt: P10300). CD8 is formed of a heterodimer between an α and β subunit linked by a pair of disulphide bonds (IntAct: P10300). The epitope recognized by clone YTS156.7.7 is restricted to the CD8β subunit and centers on Argβ77 and Argβ78) of the D-E loop of CD8β (Shore et al. 2008).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse thymocytes.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P10300 Related reagents
Entrez Gene
Cd8b1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
Regulatory
For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for CD8 Beta antibody

  1. McNicol, A.M. et al. (2007) CD8alpha/alpha homodimers fail to function as co-receptor for a CD8-dependent TCR.
    Eur J Immunol. 37 (6): 1634-41.
  2. Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
    J Mol Biol. 2008 Dec 31;384(5):1190-202.