Rat anti Mouse CD8 beta antibody, clone YTS156.7.7
recognizes the murine T-cell surface glycoprotein CD8 beta chain, also known as CD8β, LY-3 or Lyt-3. CD8β is a 213 amino acid ~25 kDa single pass type-1 transmembrane glycoprotein bearing a single Ig-like V-type
domain (UniProt: P10300
). CD8 is formed of a heterodimer between an α and β subunit linked by a pair of disulphide bonds (IntAct: P10300
). The epitope recognized by clone YTS156.7.7 is restricted to the CD8β subunit and centers on Argβ77 and Argβ78)
of the D-E loop of CD8β (Shore et al. 2008
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Mouse thymocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch.
- Entrez Gene
- GO Terms
integral to membrane
external side of plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD8 Beta antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
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Useful Reagents Available
Product Specific References
References for CD8 Beta antibody
McNicol, A.M. et al. (2007) CD8alpha/alpha homodimers fail to function as co-receptor for a CD8-dependent TCR.
Eur J Immunol. 37 (6): 1634-41.
Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
J Mol Biol. 2008 Dec 31;384(5):1190-202.