CD8 Beta antibody | YTS156.7.7
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Rat anti Mouse CD8 beta antibody, clone YTS156.7.7 recognizes the murine T-cell surface glycoprotein CD8 beta chain, also known as CD8β, LY-3 or Lyt-3. CD8β is a 213 amino acid ~25 kDa single pass type-1 transmembrane glycoprotein bearing a single Ig-like V-type domain (UniProt: P10300). CD8 is formed of a heterodimer between an α and β subunit linked by a pair of disulphide bonds (IntAct: P10300). The epitope recognized by clone YTS156.7.7 is restricted to the CD8β subunit and centers on Argβ77 and Argβ78) of the D-E loop of CD8β (Shore et al. 2008).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Mouse thymocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P10300
- Entrez Gene
- Cd8b1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
- Regulatory
- For research purposes only
Applications of CD8 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for CD8 Beta antibody
-
McNicol, A.M. et al. (2007) CD8alpha/alpha homodimers fail to function as co-receptor for a CD8-dependent TCR.
Eur J Immunol. 37 (6): 1634-41. -
Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
J Mol Biol. 2008 Dec 31;384(5):1190-202.
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