CD61 antibody | HM beta 3.1

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Hamster anti Mouse CD61:FITC

Hamster anti Mouse CD61

Hamster anti Mouse CD61:RPE

Product Type
Monoclonal Antibody
Clone
HM beta 3.1
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA2299F
Datasheet
F
MSDS
0.1 mg loader

MCA2299
Datasheet
F IP
MSDS
0.25 mg loader

MCA2299PE
Datasheet
F
MSDS
100 Tests loader

Hamster anti Mouse CD61 antibody, clone HM beta 3-1 recognizes the murine integrin beta 3 subunit (CD61), a ~90 kDa a type I membrane protein, expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptor for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.

Hamster anti Mouse CD61 antibody, clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin (Yasuda et al. 1995).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
O54890 Related reagents
Entrez Gene
Itgb3 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005178 integrin binding
GO:0007044 cell-substrate junction assembly
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007275 multicellular organismal development
GO:0009897 external side of plasma membrane
Regulatory
For research purposes only

Applications of CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/25
Immunoprecipitation
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD61 antibody

  1. Yasuda, M. et al. (1995) Expression and function of fibronectin binding integrins on rat mast cells.
    Int Immunol. 7 (2): 251-8.
  2. Hodkinson, P.S. et al. (2007) Mammalian NOTCH-1 activates beta1 integrins via the small GTPase R-Ras.
    J Biol Chem. 282 (39): 28991-9001.
  3. Moore, S.F. et al. (2015) Loss of the insulin receptor in murine megakaryocytes/platelets causes thrombocytosis and alterations in IGF signalling.
    Cardiovasc Res. 107 (1): 9-19.
  4. Kraft, S. et al. (2016) Identification and characterization of a unique role for EDB fibronectin in phagocytosis.
    J Mol Med (Berl). 94 (5): 567-81.
  5. Raouf, J. et al. (2016) mPGES-1 deletion affects platelet functions in mice.
    Clin Sci (Lond). Oct 07 [Epub ahead of print].