CD45 antibody | YW62.3
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Rat anti Mouse CD45:StarBright UltraViolet 795
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.
CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komura et al. 1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shen et al. 1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morse et al. 1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb (Charbonneau et al. 1988; Zebedee et al. 1991).
A number of different isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkeland et al. 1989).
Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45.
N.B. Some reactivity with human tissue has been observed.
- Target Species
- Product Form
- Purified IgG conjugated to StarBright UltraViolet 795 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
- Mouse spleen cells.
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright UltraViolet 795 340 792
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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References for CD45 antibody
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Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.
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Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
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Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.
Am J Pathol. 177: 2549-62.
Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.
J Neurosci. 30: 10086-95.
Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
J Immunol. 185: 2502-15.
Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.
Am J Pathol. 177: 1989-2001.
View The Latest Product References
Yang, R. et al. (2010) Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.
Nephrol Dial Transplant. 25: 2479-86.
Reed-Geaghan, E.G. et al. (2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
J Neurosci. 30: 15369-73.
Paz, H. et al. (2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
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Lee, D.C. et al. (2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.
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Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
J Neurosci. 30: 9651-8.
Long, G.G. et al. (2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.
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Drake, C. et al. (2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.
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Jawhara, S. et al. (2012) Integrin αXβz is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections.
J Immunol. 189 (5): 2468-77.
Mills, J.H. et al. (2012) A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis.
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Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.
Mol Ther. 20 (4): 808-19.
Yamauchi, S. et al. (2012) Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
FEBS Lett. 586: 3229-35.
Abramowski, D. et al. (2012) Transgenic Expression of Intraneuronal Aβ42 But Not Aβ40 Leads to Cellular Aβ Lesions, Degeneration, and Functional Impairment without Typical Alzheimer's Disease Pathology.
J Neurosci. 32: 1273-83.
Chu, C.J. et al. (2013) Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography.
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Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
Invest Ophthalmol Vis Sci. 55 (1): 247-58.
Yazid, S. et al. (2015) Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease.
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Carbajal, K.S. et al. (2015) Th Cell Diversity in Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis.
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- Entrez Gene
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0051726 regulation of cell cycle
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007159 leukocyte cell-cell adhesion
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0031953 negative regulation of protein autophosphorylation
- GO:0034113 heterotypic cell-cell adhesion
- GO:0042098 T cell proliferation
- GO:0042100 B cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045059 positive thymic T cell selection
- GO:0045060 negative thymic T cell selection
- GO:0045121 membrane raft
- GO:0045577 regulation of B cell differentiation
- GO:0045588 positive regulation of gamma-delta T cell differentiation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050855 regulation of B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
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