CD4 antibody | RM4-5
Rat anti Mouse CD4 antibody, clone RM4-5 (MCA2691) used to identify T helper lymphocytes in murine brain frontal cortex by immunohistochemistry on floating sections.
Image caption:
TDP-43 overexpression induces blood-brain barrier permeability. a Representative images depicting CD3+ T cells in the frontal cortex of mice. Scale bar = 10 μm. b Manual count of CD3+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001). c Representative images depicting CD4+ T cells in the frontal cortex of mice. Scale bar = 10 &mum;m. d Manual count of CD4+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group; ***p <0.001). e Representative images depicting mouse IgG in the frontal cortex of mouse tissue. Scale bar = 20 μm. f Quantification of percent area of the frontal cortex covered by mouse IgG staining. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001).
From: Zamudio F, Loon AR, Smeltzer S, Benyamine K, Navalpur Shanmugam NK, Stewart NJF, Lee DC, Nash K, Selenica MB.
TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 2020 Sep 26;17(1):283.
doi: 10.1186/s12974-020-01952-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C ResetRat anti Mouse CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- RM4-5
- Isotype
- IgG2a
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- BALB/c mouse thymocytes
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
NEAT | 1/2 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rat IgG2a Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse Seroblock FcR | ||||||
References for CD4 antibody
-
von Kutzleben, S. et al. (2017) Depletion of CD52-positive cells inhibits the development of central nervous system autoimmune disease, but deletes an immune-tolerance promoting CD8 T-cell population. Implications for secondary autoimmunity of alemtuzumab in multiple sclerosis.
Immunology. 150 (4): 444-55. -
Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 17 (1): 283.
Further Reading
-
Fehérvari, Z. & Sakaguchi, S. (2004) CD4+ Tregs and immune control.
J Clin Invest. 114 (9): 1209-17.
- Synonyms
- L3T4 Antigen
- LY-4
- RRID
- AB_905959
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- View More GO Terms
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
MCA2691PE
LINN0915
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD4 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up