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CD39 antibody | Duha59

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Rat anti Mouse CD39:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
Duha59
Isotype
IgG2a
Specificity
CD39

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA4714A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Rat anti Mouse CD39 antibody, clone Duha59 recognizes CD39, also known as Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or Ecto-ATP diphosphohydrolase. CD39 was first described as a B cell activation marker, but is also expressed on natural killer cells, monocytes, dendritic cells and subsets of activated T cells. It is critical in the regulation of leukocyte accumulation within hypoxic tissues and plays a role in the thromboregulation of platelet and endothelial function activation. CD39, along with CD73, distinguishes regulatory T cells from other resting or activated T cells and plays a role in the suppressive action of regulatory T cells.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
A CD39 expression construct and a final boost with CD39-transfected HEK cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488 MCA1212A488 F 100 Tests/1ml loader
List Price Your Price
loader
Description Rat IgG2a Negative Control:Alexa Fluor® 488

Further Reading

  1. Dwyer, K.M. et al. (2007) CD39 and control of cellular immune responses.
    Purinergic Signal. 3 (1-2): 171-80.

RRID
AB_2099924
UniProt
P55772
Entrez Gene
Entpd1
GO Terms
GO:0017110 nucleoside-diphosphatase activity
GO:0017111 nucleoside-triphosphatase activity
GO:0016021 integral to membrane
GO:0005524 ATP binding
GO:0005605 basal lamina
GO:0006200 ATP catabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009181 purine ribonucleoside diphosphate catabolic process
GO:0030168 platelet activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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