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CD38 antibody | 90

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Anti Mouse CD38 Antibody, clone 90 thumbnail image 1
Anti Mouse CD38 Antibody, clone 90 gallery image 1
Figure A. Alexa Fluor 700 conjugated rat anti mouse CD19 (MCA1439A700) and FITC conjugated rat IgG2a isotype control (MCA1212F). Figure B. Alexa Fluor 700 conjugated rat anti mouse CD19 (MCA1439A700) and FITC conjugated rat anti mouse CD38 (MCA1511FT). All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Rat anti Mouse CD38:RPE

Product Type
Monoclonal Antibody
Clone
90
Isotype
IgG2a
Specificity
CD38

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Product Code Applications Pack Size List Price Your Price Qty
MCA1511PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse CD38 antibody, clone 90 recognizes the murine CD38 cell surface antigen, a ~42-46kDa glycoprotein expressed by B lymphocytes, a proportion of peripheral T lymphocytes and by peritoneal macrophages.

In the mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
sucrose
Immunogen
Mouse pre-B cells derived from IL-7 dependent bone marrow cultures.
Fusion Partners
Lymph node cells from an immunized rat were fused with cells of the mouse P3X63Ag.8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl,
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
>
List Price Your Price
Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD38 antibody

  1. Lund, F. et al. (1995) Murine CD38: an immunoregulatory ectoenzyme.
    Immunol Today. 16 (10): 469-73.
  2. Oliver, A.M. et al. (1997) Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.
    J Immunol. 158 (3): 1108-15.

RRID
AB_321683
UniProt
P56528
Entrez Gene
Cd38
GO Terms
GO:0003953 NAD+ nucleosidase activity
GO:0016021 integral to membrane
GO:0005488 binding
GO:0009986 cell surface
GO:0016849 phosphorus-oxygen lyase activity
GO:0030890 positive regulation of B cell proliferation
View more products with CD38 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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