CD22 antibody | OX-97

Rat anti Mouse CD22:RPE

Product Type
Monoclonal Antibody
Clone
OX-97
Isotype
IgG1
Specificity
CD22

Product Code Applications Pack Size List Price Your Price Qty
MCA2187PE
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Rat anti Mouse CD22 antibody, clone OX-97 recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD22 antibody

  1. Bakker, T.R. et al. (2002) Comparison of CD22 binding to native CD45 and synthetic oligosaccharide.
    Eur J Immunol. 32 (7): 1924-32.
  2. Duong, B.H. et al. (2010) Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.
    J Immunol. 184: 4143-58.

Synonyms
Lyb-8
RRID
AB_323529
UniProt
P35329
Entrez Gene
Cd22
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0015026 coreceptor activity
View more products with CD22 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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