CD178 antibody | MFL4
Hamster anti Mouse CD178:Alexa Fluor®647
- Product Type
- Monoclonal Antibody
- Clone
- MFL4
- Isotype
- IgG
- Specificity
- CD178
Antibody Quality and Performance Guarantee
| Hamster anti Mouse CD178 antibody, clone MFL4 recognizes both mouse and rat CD178, otherwise known as Fas Ligand/ CD95L, a 40 kDa type II transmembrane glycoprotein and member of the TNF/NGF superfamily, expressed by activated T cells and NK cells, which can be induced on a variety of cells by radiation, heat shock, chemotherapeutic agents and viral infection. CD178 acts as a key effector of cytotoxicity and in the regulation of immune responses. The binding of CD178 to its receptor CD95 (Fas), induces Fas-mediated apoptosis of target cells, and may be involved in the induction of peripheral tolerance and neutrophil chemotaxis. The binding of decoy receptor 3 (DcR3) to CD178 has been shown to inhibit CD178-mediated apoptosis. Hamster anti Mouse CD178 antibody, clone MFL4 is reported to block CD178/CD95 induced apoptosis. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- B6 mouse FasL/BHK cells
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | MCA2356A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
Source Reference
-
Kayagaki, N. et al. (1997) Polymorphism of murine Fas ligand that affects the biological activity.
Proc Natl Acad Sci U S A. 94 (8): 3914-9.
References for CD178 antibody
-
Trinité, B. et al. (2000) A subset of cytolytic dendritic cells in rat.
J Immunol. 165 (8): 4202-8.
- Synonyms
- FAS Ligand
- RRID
- AB_10673435
- UniProt
- P41047
- Entrez Gene
- Fasl
- GO Terms
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
- GO:0046666 retinal cell programmed cell death
MCA2896A647
154481
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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