CD117 antibody | 2B8

Rat anti Mouse CD117:StarBright Violet 475

Product Type
Monoclonal Antibody
Clone
2B8
Isotype
IgG2b
Specificity
CD117

Product Code Applications Pack Size List Price Your Price Qty
MCA1365SBV475
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD117 antibody, clone 2B8 recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.

Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Violet 475 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
IL-3 dependent mast cells.
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 475 405 479
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

Source Reference

  1. Ikuta, K. & Weissman, I.L. (1992) Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
    Proc Natl Acad Sci U S A. 89 (4): 1502-6.

Further Reading

  1. Fleming WH et al. (1993) Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo.
    Proc Natl Acad Sci U S A. 90 (8): 3760-4.
  2. Rico-Vargas, S.A. et al. (1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.
    J Immunol. 152 (6): 2845-52.
  3. Manova, K. et al. (1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.
    J Neurosci. 12 (12): 4663-76.
  4. Maeda, H. et al. (1992) Requirement of c-kit for development of intestinal pacemaker system.
    Development. 116 (2): 369-75.
  5. Takagi, M. et al. (1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.
    J Immunol. 148 (11): 3446-53.
  6. de Vries, P. et al. (1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.
    J Exp Med. 176 (6): 1503-9.

Synonyms
C-KIT
SCF-R
UniProt
P05532
Entrez Gene
Kit
GO Terms
GO:0005515 protein binding
GO:0002318 myeloid progenitor cell differentiation
GO:0002320 lymphoid progenitor cell differentiation
GO:0002573 myeloid leukocyte differentiation
GO:0016021 integral to membrane
GO:0005020 stem cell factor receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0006687 glycosphingolipid metabolic process
GO:0006935 chemotaxis
GO:0007243 intracellular protein kinase cascade
GO:0007286 spermatid development
GO:0008284 positive regulation of cell proliferation
GO:0009314 response to radiation
GO:0009897 external side of plasma membrane
GO:0010628 positive regulation of gene expression
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0019221 cytokine-mediated signaling pathway
GO:0035234 germ cell programmed cell death
GO:0043069 negative regulation of programmed cell death
GO:0043406 positive regulation of MAP kinase activity
GO:0046777 protein autophosphorylation
GO:0048066 developmental pigmentation
GO:0048070 regulation of developmental pigmentation
View more products with CD117 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products