Vesicle-fusing ATPase antibody | CD01/1F3

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Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with vesicle-fusing ATPase antibody (1/2000, **VMA00942**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to vesicle-fusing ATPase (molecular weight 80 kDa)

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of NSF from Jurkat whole cell lysate. The Input lane is 10% input; the NSF-IP lane is the immunoprecipitation with the NSF antibody (2.5 µg, **VMA00942**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA929). Western blot analysis was performed using NSF antibody (1/1000, **VMA00942**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to NSF.

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Vesicle-fusing ATPase (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Vesicle-fusing ATPase antibody (**VMA00942**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 14 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti Vesicle-fusing ATPase Antibody, clone CD01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 4 — Normalized signal intensity of Vesicle-fusing ATPase from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Vesicle-fusing ATPase (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB IP

Mouse anti Vesicle-fusing ATPase

Product Type: PrecisionAb Monoclonal
Clone: CD01/1F3
Isotype: IgG2a
Format: Purified
Specificity: Vesicle-fusing ATPase

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VMA00942 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Vesicle-fusing ATPase antibody recognizes vesicle-fusing ATPase, also known as NEM-sensitive fusion protein (NSF). Vesicle-fusing ATPase is an ATPase that plays a role in vesicular traffic by associating with SNAP and the membrane-fusing SNARE proteins, forming the 20S complex important for membrane trafficking (Nishizaki et al. 2016). Vesicle-fusing ATPase in combination with SNAPs is able to disassemble the SNARE complex, leading to release of neurotransmitters and facilitating other membrane trafficking processes (Choi et al. 2018). In addition, vesicle-fusing ATPase is responsible for regenerating active SNAREs after fusion, ready for the next round of fusion. In neurons, inactivation of vesicle-fusing ATPase results in a huge buildup of Golgi fragments, transport vesicles, and late endosomes, followed by delayed neuronal death (Yuan et al. 2019). Defects in membrane trafficking have been reported in a range of human diseases including cancer, diabetes, neurodegenerative disorders, ciliopathies, and infections (Yoon et al. 2018).

Target Species: Human
Western Blotting: Mouse anti vesicle-fusing ATPase detects a band of approximately 80 kDa in Jurkat cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 500-744 of human vesicle-fusing ATPase
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P46459
Entrez Gene: NSF
GO Terms: GO:0004252 serine-type endopeptidase activity; GO:0004176 ATP-dependent peptidase activity; GO:0005829 cytosol; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0006508 proteolysis; GO:0007268 synaptic transmission; GO:0015031 protein transport