Vesicle-fusing ATPase antibody | CD01/1F3

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Mouse anti Vesicle-fusing ATPase

Product Type
PrecisionAb Monoclonal
Clone
CD01/1F3
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00942
Datasheet
IP WB
SDS
100 µl loader

Mouse anti Vesicle-fusing ATPase antibody recognizes vesicle-fusing ATPase, also known as NEM-sensitive fusion protein (NSF). Vesicle-fusing ATPase is an ATPase that plays a role in vesicular traffic by associating with SNAP and the membrane-fusing SNARE proteins, forming the 20S complex important for membrane trafficking (Nishizaki et al. 2016). Vesicle-fusing ATPase in combination with SNAPs is able to disassemble the SNARE complex, leading to release of neurotransmitters and facilitating other membrane trafficking processes (Choi et al. 2018). In addition, vesicle-fusing ATPase is responsible for regenerating active SNAREs after fusion, ready for the next round of fusion. In neurons, inactivation of vesicle-fusing ATPase results in a huge buildup of Golgi fragments, transport vesicles, and late endosomes, followed by delayed neuronal death (Yuan et al. 2019). Defects in membrane trafficking have been reported in a range of human diseases including cancer, diabetes, neurodegenerative disorders, ciliopathies, and infections (Yoon et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti vesicle-fusing ATPase detects a band of approximately 80 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 500-744 of human vesicle-fusing ATPase
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P46459
Entrez Gene
NSF
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0004176 ATP-dependent peptidase activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006508 proteolysis
GO:0007268 synaptic transmission
GO:0015031 protein transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Vesicle-fusing ATPase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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