VCP antibody

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Mouse anti VCP

VCP antibody recognizes transitional endoplasmic reticulum ATPase, also known as 15S Mg(2+)-ATPase p97 subunit, TER ATPase, epididymis luminal protein 220, epididymis secretory protein Li 70, valosin-containing protein or yeast CDC48p homolog.

VCP Antibody with Control Lysate

VCP antibody recognizes transitional endoplasmic reticulum ATPase, also known as 15S Mg(2+)-ATPase p97 subunit, TER ATPase, epididymis luminal protein 220, epididymis secretory protein Li 70, valosin-containing protein or yeast CDC48p homolog.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00307 WB 100 µl
VMA00307KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human VCP antibody recognizes the valosin-containing protein (VCP), also known as transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, epididymis luminal protein 220, epididymis secretory protein Li 70, transitional endoplasmic reticulum ATPase, and yeast Cdc48p homolog.

Encoded by VCP gene, valosin-containing protein is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation (provided by RefSeq, Jul 2008)

Mouse anti Human VCP antibody detects a band of 89 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti VCP detects a band of approximately 89 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human VCP
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P55072 Related reagents
Entrez Gene
VCP Related reagents
GO Terms
GO:0000502 proteasome complex
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0016567 protein ubiquitination
GO:0005634 nucleus
GO:0005792 microsome
GO:0006302 double-strand break repair
GO:0008289 lipid binding
GO:0006919 activation of caspase activity
GO:0016887 ATPase activity
GO:0019903 protein phosphatase binding
GO:0019904 protein domain specific binding
GO:0030433 ER-associated protein catabolic process
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0030970 retrograde protein transport, ER to cytosol
GO:0031593 polyubiquitin binding
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of VCP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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