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VCP antibody

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Anti VCP Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti VCP Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with VCP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to VCP (molecular weight 89 kDa)

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WB

Mouse anti VCP

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
VCP

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Product Code Applications Pack Size List Price Your Price Qty
VMA00307
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human VCP antibody recognizes the valosin-containing protein (VCP), also known as transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, epididymis luminal protein 220, epididymis secretory protein Li 70, transitional endoplasmic reticulum ATPase, and yeast Cdc48p homolog.

Encoded by VCP gene, valosin-containing protein is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation (provided by RefSeq, Jul 2008)

Mouse anti Human VCP antibody detects a band of 89 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti VCP detects a band of approximately 89 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human VCP
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P55072
Entrez Gene
VCP
GO Terms
GO:0000502 proteasome complex
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0016567 protein ubiquitination
GO:0005634 nucleus
GO:0005792 microsome
GO:0006302 double-strand break repair
GO:0008289 lipid binding
GO:0006919 activation of caspase activity
GO:0016887 ATPase activity
GO:0019903 protein phosphatase binding
GO:0019904 protein domain specific binding
GO:0030433 ER-associated protein catabolic process
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0030970 retrograde protein transport, ER to cytosol
GO:0031593 polyubiquitin binding
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process

VMA00307

151204

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