VCP antibody

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Anti VCP Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with VCP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to VCP (molecular weight 89 kDa)

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Mouse anti VCP

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: VCP

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VMA00307 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human VCP antibody recognizes the valosin-containing protein (VCP), also known as transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, epididymis luminal protein 220, epididymis secretory protein Li 70, transitional endoplasmic reticulum ATPase, and yeast Cdc48p homolog.

Encoded by VCP gene, valosin-containing protein is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation (provided by RefSeq, Jul 2008)

Mouse anti Human VCP antibody detects a band of 89 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti VCP detects a band of approximately 89 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human VCP
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P55072
Entrez Gene: VCP
GO Terms: GO:0000502 proteasome complex; GO:0005829 cytosol; GO:0005783 endoplasmic reticulum; GO:0005524 ATP binding; GO:0016567 protein ubiquitination; GO:0005634 nucleus; GO:0005792 microsome; GO:0006302 double-strand break repair; GO:0008289 lipid binding; View More GO Terms; GO:0006919 activation of caspase activity; GO:0016887 ATPase activity; GO:0019903 protein phosphatase binding; GO:0019904 protein domain specific binding; GO:0030433 ER-associated protein catabolic process; GO:0030968 endoplasmic reticulum unfolded protein response; GO:0030970 retrograde protein transport, ER to cytosol; GO:0031593 polyubiquitin binding; GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process