VASP antibody | OTI1C3

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Mouse anti VASP

Product Type
PrecisionAb Monoclonal
Clone
OTI1C3
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00326
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human VASP antibody recognizes the vasodilator-stimulated phosphoprotein (VASP).

VASP is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG (provided by RefSeq, Jul 2008).

Mouse anti Human VASP antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti VASP detects a band of approximately 50 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human VASP (NP_003361) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P50552 Related reagents
Entrez Gene
VASP Related reagents
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005522 profilin binding
GO:0005925 focal adhesion
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017124 SH3 domain binding
GO:0031258 lamellipodium membrane
GO:0031527 filopodium membrane
GO:0034329 cell junction assembly
GO:0050852 T cell receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of VASP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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