USP8 antibody | AB01/1B5

100% Secure


Mouse anti USP8

Product Type
PrecisionAb Monoclonal
Clone
AB01/1B5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00973
Datasheet
IP WB
SDS
100 µl loader

Mouse anti USP8 antibody recognizes ubiquitin carboxyl-terminal hydrolase 8, also known as deubiquitinating enzyme 8 and hUBPy. USP8 is a ubiquitin-specific protease and essential deubiquitinating enzyme which is known to participate in protein trafficking and receptor tyrosine kinase degradation. It is involved with deubiquitination of EGFR, but the ubiquitination, lysosomal trafficking and/or stability of other transmembrane proteins have also been shown to be regulated by USP8 (Dufner and Knobeloch 2019). USP8 has been found to regulate mitophagy, and may regulate several components of the autophagy pathway (Peng et al. 2020). Gain-of-function mutations in USP8 are associated with Cushing disease, a condition in which the pituitary gland releases too much adrenocorticotropic hormone (ACTH) (Pivonello et al. 2015). USP8 has been linked to the development of several tumor types, including breast cancer, lung cancer, bladder cancer, and cervical cancer (Sun et al. 2020).

Product Details

Target Species
Human
Western Blotting
Mouse anti USP8 detects a band of approximately 141 kDa in MCF-7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 600-1118 of human USP8
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P40818
Entrez Gene
USP8
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005829 cytosol
GO:0004843 ubiquitin-specific protease activity
GO:0005634 nucleus
GO:0005769 early endosome
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007032 endosome organization
GO:0007049 cell cycle
GO:0008283 cell proliferation
GO:0010008 endosome membrane
GO:0017124 SH3 domain binding
GO:0019897 extrinsic to plasma membrane
GO:0070536 protein K63-linked deubiquitination
GO:0071108 protein K48-linked deubiquitination
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of USP8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra