Troponin T antibody

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Mouse anti Troponin T (Cardiac)

Troponin T antibody recognizes the cardiac muscle protein troponin T, also known as dilated 1D (autosomal dominant), hypertrophic 2 or troponin T2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00101 WB 100 µl
Mouse anti Human troponin T antibody recognizes troponin T, cardiac muscle, also known as cardiomyopathy dilated 1D (autosomal dominant) and cardiomyopathy hypertrophic 2.

Encoded by the TNNT2 gene, troponin T is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in TNNT2 have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for TNNT2 undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined (provided by RefSeq, Jul 2008).

Mouse anti Human troponin T antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti troponin T detects a band of approximately 40 kDa in human heart cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified native Troponin T protein from human cardiac tissue.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P45379 Related reagents
Entrez Gene
TNNT2 Related reagents
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005523 tropomyosin binding
GO:0005861 troponin complex
GO:0008016 regulation of heart contraction
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031013 troponin I binding
GO:0032780 negative regulation of ATPase activity
GO:0032781 positive regulation of ATPase activity
GO:0051592 response to calcium ion
GO:0055010 ventricular cardiac muscle tissue morphogenesis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Troponin T antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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