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Thymidylate Synthase antibody | OTI9H4

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Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human thymidylate synthase antibody, clone OTI9H4 (VMA00217) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to thymidylate synthase (molecular weight 30 kDa).
Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of thymidylate synthase from Jurkat whole cell lysate. The Input lane is 10% input; the THYMIDYLATE SYNTHASE-IP lane is the immunoprecipitation with the thymidylate synthase antibody (2.5 µg, VMA00217); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using thymidylate synthase antibody (1/1,000, VMA00217) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to thymidylate synthase.

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WB IP

Mouse anti Thymidylate Synthase

Product Type
PrecisionAb Monoclonal
Clone
OTI9H4
Isotype
IgG1
Format
Purified
Specificity
Thymidylate Synthase

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VMA00217
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Mouse anti Human thymidylate synthase antibody, clone OTI9H4 recognizes thymidylate synthase, also known as TSase.

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of TYMS and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).

Mouse anti Human thymidylate synthase antibody, clone OTI9H4 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti thymidylate synthase detects a band of approximately 30 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human thymidylate synthase (NP_001062) produced in SF9 cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P04818
Entrez Gene
TYMS
GO Terms
GO:0006260 DNA replication
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004799 thymidylate synthase activity
GO:0006206 pyrimidine base metabolic process
GO:0006281 DNA repair
GO:0046683 response to organophosphorus
GO:0048015 phosphatidylinositol-mediated signaling
GO:0046134 pyrimidine nucleoside biosynthetic process
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