Thymidylate Synthase antibody | OTI9H4

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Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human thymidylate synthase antibody, clone OTI9H4 (**VMA00217**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to thymidylate synthase (molecular weight 30 kDa).

Anti Thymidylate Synthase Antibody, clone OTI9H4 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of thymidylate synthase from Jurkat whole cell lysate. The Input lane is 10% input; the THYMIDYLATE SYNTHASE-IP lane is the immunoprecipitation with the thymidylate synthase antibody (2.5 µg, **VMA00217**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using thymidylate synthase antibody (1/1,000, **VMA00217**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to thymidylate synthase.

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Mouse anti Thymidylate Synthase

Product Type: PrecisionAb Monoclonal
Clone: OTI9H4
Isotype: IgG1
Format: Purified
Specificity: Thymidylate Synthase

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VMA00217 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human thymidylate synthase antibody, clone OTI9H4 recognizes thymidylate synthase, also known as TSase.

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of TYMS and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).

Mouse anti Human thymidylate synthase antibody, clone OTI9H4 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti thymidylate synthase detects a band of approximately 30 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human thymidylate synthase (NP_001062) produced in SF9 cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P04818
Entrez Gene: TYMS
GO Terms: GO:0006260 DNA replication; GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0005829 cytosol; GO:0004799 thymidylate synthase activity; GO:0006206 pyrimidine base metabolic process; GO:0006281 DNA repair; GO:0046683 response to organophosphorus; GO:0048015 phosphatidylinositol-mediated signaling; GO:0046134 pyrimidine nucleoside biosynthetic process; View More GO Terms