TCF3 antibody | CD01/1E7
Mouse anti TCF3 antibody recognizes transcription factor E2-alpha, also known as BHLHB21, E2A, and ITF1.
TCF3 is a member of the E protein (class 1) family of helix-loop-helix transcription factors. The protein plays important roles in maintenance and differentiation of stem cells, and is thought to be an important regulator of stem cell identity. Accordingly, deletion of the gene encoding TCF3 leads to high expression of pluripotency genes and delays differentiation of embryonic stem cells (Yamazaki et al. 2018). TCF3 is required for survival and tumorigenicity of colorectal cancer cells (Taniue et al. 2016). The fusion protein TCF3-PBX1 is an oncogenic transcription factor, occuring in a subset of acute lymphoblastic leukemias (Eldfors et al. 2017). In addition, as a chimeric transcription factors, TCF3-HLF defines an incurable subtype of acute lymphoblastic leukemia (Huang et al. 2019).
TCF3 is a member of the E protein (class 1) family of helix-loop-helix transcription factors. The protein plays important roles in maintenance and differentiation of stem cells, and is thought to be an important regulator of stem cell identity. Accordingly, deletion of the gene encoding TCF3 leads to high expression of pluripotency genes and delays differentiation of embryonic stem cells (Yamazaki et al. 2018). TCF3 is required for survival and tumorigenicity of colorectal cancer cells (Taniue et al. 2016). The fusion protein TCF3-PBX1 is an oncogenic transcription factor, occuring in a subset of acute lymphoblastic leukemias (Eldfors et al. 2017). In addition, as a chimeric transcription factors, TCF3-HLF defines an incurable subtype of acute lymphoblastic leukemia (Huang et al. 2019).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti TCF3 antibody detects a band of approximately 70 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 100-654 of human TCF3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15923
- Entrez Gene
- TCF3
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0002326 B cell lineage commitment
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005667 transcription factor complex
- GO:0008134 transcription factor binding
- GO:0042802 identical protein binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016563 transcription activator activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0033152 immunoglobulin V(D)J recombination
- GO:0042692 muscle cell differentiation
- GO:0042803 protein homodimerization activity
- GO:0043234 protein complex
- GO:0043425 bHLH transcription factor binding
- GO:0045787 positive regulation of cell cycle
- GO:0046982 protein heterodimerization activity
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0070644 vitamin D response element binding
- GO:0070888 E-box binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of TCF3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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