TCF3 antibody | CD01/1E7

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Anti TCF3 Antibody, clone CD01/1E7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TCF3 antibody (1/1000, **VMA00808**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to TCF3 (molecular weight 70 kDa)

Mouse anti TCF3

Product Type: PrecisionAb Monoclonal
Clone: CD01/1E7
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00808 Datasheet	IP WB SDS	100 µl	Log in		Get Quote

Mouse anti TCF3 antibody recognizes transcription factor E2-alpha, also known as BHLHB21, E2A, and ITF1.

TCF3 is a member of the E protein (class 1) family of helix-loop-helix transcription factors. The protein plays important roles in maintenance and differentiation of stem cells, and is thought to be an important regulator of stem cell identity. Accordingly, deletion of the gene encoding TCF3 leads to high expression of pluripotency genes and delays differentiation of embryonic stem cells (Yamazaki et al. 2018). TCF3 is required for survival and tumorigenicity of colorectal cancer cells (Taniue et al. 2016). The fusion protein TCF3-PBX1 is an oncogenic transcription factor, occuring in a subset of acute lymphoblastic leukemias (Eldfors et al. 2017). In addition, as a chimeric transcription factors, TCF3-HLF defines an incurable subtype of acute lymphoblastic leukemia (Huang et al. 2019).

Product Details

Target Species

Human

Western Blotting

Mouse anti TCF3 antibody detects a band of approximately 70 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - Liquid

Preparation

Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

Immunogen

E. coli-derived recombinant protein of amino acids 100-654 of human TCF3

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: P15923
Entrez Gene: TCF3
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0002326 B cell lineage commitment; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0005667 transcription factor complex; GO:0008134 transcription factor binding; GO:0042802 identical protein binding; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0016563 transcription activator activity; GO:0030890 positive regulation of B cell proliferation; GO:0033152 immunoglobulin V(D)J recombination; GO:0042692 muscle cell differentiation; GO:0042803 protein homodimerization activity; GO:0043234 protein complex; GO:0043425 bHLH transcription factor binding; GO:0045787 positive regulation of cell cycle; GO:0046982 protein heterodimerization activity; GO:0051149 positive regulation of muscle cell differentiation; GO:0070644 vitamin D response element binding; GO:0070888 E-box binding
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of TCF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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