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TCF3 antibody | CD01/1E7

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Anti TCF3 Antibody, clone CD01/1E7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti TCF3 Antibody, clone CD01/1E7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti TCF3 Antibody, clone CD01/1E7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with TCF3 antibody (1/1000, VMA00808) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to TCF3 (molecular weight 70 kDa)
Anti TCF3 Antibody, clone CD01/1E7 (PrecisionAb Monoclonal Antibody) gallery image 2
Mouse anti Human TCF3 (VMA00808) (green) and Mouse IgG1 isotype control (MCA928) (blue) detected with FITC conjugated Goat anti Mouse IgG1 (STAR132F). Secondary only control shown in orange and unstained cells in black. All experiments performed on fixed and permeabilized HeLa cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

Mouse anti TCF3

Product Type
PrecisionAb Monoclonal
Clone
CD01/1E7
Isotype
IgG1
Format
Purified
Specificity
TCF3

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Product Code Applications Pack Size List Price Your Price Qty
VMA00808
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SDS Safety Datasheet SDS
F IP WB 100 µl loader
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loader

Mouse anti TCF3 antibody recognizes transcription factor E2-alpha, also known as BHLHB21, E2A, and ITF1.

TCF3 is a member of the E protein (class 1) family of helix-loop-helix transcription factors. The protein plays important roles in maintenance and differentiation of stem cells, and is thought to be an important regulator of stem cell identity. Accordingly, deletion of the gene encoding TCF3 leads to high expression of pluripotency genes and delays differentiation of embryonic stem cells (Yamazaki et al. 2018). TCF3 is required for survival and tumorigenicity of colorectal cancer cells (Taniue et al. 2016). The fusion protein TCF3-PBX1 is an oncogenic transcription factor, occuring in a subset of acute lymphoblastic leukemias (Eldfors et al. 2017). In addition, as a chimeric transcription factors, TCF3-HLF defines an incurable subtype of acute lymphoblastic leukemia (Huang et al. 2019).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti TCF3 antibody detects a band of approximately 70 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 100-654 of human TCF3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P15923
Entrez Gene
TCF3
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0002326 B cell lineage commitment
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005667 transcription factor complex
GO:0008134 transcription factor binding
GO:0042802 identical protein binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016563 transcription activator activity
GO:0030890 positive regulation of B cell proliferation
GO:0033152 immunoglobulin V(D)J recombination
GO:0042692 muscle cell differentiation
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0043425 bHLH transcription factor binding
GO:0045787 positive regulation of cell cycle
GO:0046982 protein heterodimerization activity
GO:0051149 positive regulation of muscle cell differentiation
GO:0070644 vitamin D response element binding
GO:0070888 E-box binding

VMA00808

100004329

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