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TATA-BOX-BINDING Protein antibody | 830CT4.3.3

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KO Validated
IP-MS Validated
Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with TATA-box-binding protein antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to TATA-box-binding protein (molecular weight 40 kDa)
Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of TATA-box-binding protein knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti TATA-box-binding protein antibody (VMA00301) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 41 second and (B) 30 second exposure.
Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of TATA-box-binding protein from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti TATA-BOX-BINDING Protein Antibody, clone 830CT4.3.3 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of TATA-box-binding protein from HEK293 whole cell lysate. The Input lane is 10% input; the TBP-IP lane is the immunoprecipitation with the TATA-box-binding protein antibody (2.5 µg, VMA00301); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using TATA-box-binding protein antibody (1/1,000, VMA00301) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to TATA-box-binding protein.

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WB IP

Mouse anti TATA-BOX-BINDING Protein

Product Type
PrecisionAb Monoclonal
Clone
830CT4.3.3
Isotype
IgG1
Format
Purified
Specificity
TATA-BOX-BINDING Protein

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VMA00301
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IP WB 100 µl loader
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Mouse anti Human TATA-box-binding protein antibody recognizes the TATA-box-binding protein, also known as TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor and transcription initiation factor TFIID TBP subunit.

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TBP gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for TBP (provided by RefSeq, Feb 2010)

Mouse anti Human TATA-box-binding protein antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti TATA-box-binding protein detects a band of approximately 40 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified His-tagged TATA-box-binding protein
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P20226
Entrez Gene
TBP
GO Terms
GO:0005669 transcription factor TFIID complex
GO:0005672 transcription factor TFIIA complex
GO:0006355 regulation of transcription, DNA-dependent
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006383 transcription from RNA polymerase III promoter
GO:0008219 cell death
GO:0016032 viral reproduction
GO:0010843 promoter binding
GO:0016251 general RNA polymerase II transcription factor activity
GO:0032568 general transcription from RNA polymerase II promoter
GO:0044419 interspecies interaction between organisms
GO:0070491 repressing transcription factor binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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