SSB antibody | OTI1E11

100% Secure


Mouse anti SSB

SSB antibody recognizes the lupus La protein, also known as La ribonucleoprotein domain family member 3, SS-B, SS-B/La protein, La autoantigen or sjoegren syndrome type B antigen. This antibody has been extensively validated for western blotting using whole cell lysates.

SSB Antibody with Control Lysate

SSB antibody recognizes the lupus La protein, also known as La ribonucleoprotein domain family member 3, SS-B, SS-B/La protein, La autoantigen or sjoegren syndrome type B antigen. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1E11
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00112 WB 100 µl
VMA00112KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SSB antibody recognizes SSB, also known as La ribonucleoprotein domain family, member 3, SS-B/La protein, autoantigen La, la autoantigen, lupus La antigen, lupus La protein or sjoegren syndrome type B antigen.

The protein encoded by the SSB gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus (provided by RefSeq, Nov 2011).

Mouse anti Human SSB antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full-length protein expressed in 293T cell transfected with human SSB expression vector

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P05455 Related reagents
Entrez Gene
SSB Related reagents
GO Terms
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003729 mRNA binding
GO:0030529 ribonucleoprotein complex
GO:0005634 nucleus
GO:0006400 tRNA modification
GO:0008334 histone mRNA metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SSB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SSB detects a band of approximately 50 kDa in HEK293 cell lysates.
Western Blotting
Anti SSB detects a band of approximately 50 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB