SRSF3 antibody | CD01/2F12

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Mouse anti SRSF3

Product Type
PrecisionAb Monoclonal
Clone
CD01/2F12
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00852
Datasheet
WB
SDS
100 µl loader

Mouse anti SRSF3 antibody recognizes serine/arginine-rich splicing factor 3, also known as SFRS3, SRP20.

SRSF3 is the smallest member of the SR family of proteins, and promotes RNA splicing by recruiting spliceosome components. In addition, SRSF3 appears to regulate cell proliferation by controlling the G2/M transition (Kumar et al. 2019), and achieves this by regulating expression of FOXM1 and two of its transcriptional targets, PLK1 and Cdc25B (Kuranaga et al. 2018). Via alternative splicing, the protein can promote cellular senescence which is important for preventing cancer and contributing to aging (Shen et al. 2019). SRSF3 has been suggested to be a proto-oncogene, and is overexpressed in a number of tumors including breast, colon, ovarian, cervical, bone cancer, and glioma (Song et al. 2020). SRSF3 is also essential for development of fertilized-competent oocytes (Vinh Do et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti SRSF3 antibody detects a band of approximately 23 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-164 of human SRSF3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P84103
Entrez Gene
SRSF3
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0005654 nucleoplasm
GO:0006369 termination of RNA polymerase II transcription
GO:0006406 mRNA export from nucleus
GO:0031124 mRNA 3'-end processing
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SRSF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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