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Sp1 antibody | 1326CT463.109.176

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Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Sp1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to Sp1 (molecular weight 90 kDa)
Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of Sp1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti Sp1 antibody (VMA00309) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 62 second and (B) 30 second exposure.
Anti Sp1 Antibody, clone 1326CT463.109.176 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of Sp1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti Sp1

Product Type
PrecisionAb Monoclonal
Clone
1326CT463.109.176
Isotype
IgG1
Format
Purified
Specificity
Sp1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00309
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human Sp1 antibody recognizes the transcription factor Sp1, also known as specificity protein 1.

Encoded by the SP1 gene, transcription factor Sp1 is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for SP1 (provided by RefSeq, Oct 2011).

Mouse anti Human Sp1 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti Sp1 detects a band of approximately 90 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombination human Sp1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P08047
Entrez Gene
SP1
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008022 protein C-terminus binding
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0042803 protein homodimerization activity
GO:0042826 histone deacetylase binding
GO:0043923 positive regulation by host of viral transcription
GO:0045817 positive regulation of transcription from RNA polymerase II promoter, global

VMA00309

151204

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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