SOX9 antibody | OTI2H10
Mouse anti SOX9
SOX9 antibody recognizes the transcription factor SOX-9, also known as SRY (sex-determining region Y)-box 9 protein or SRY-related HMG-box, gene 9. This antibody has been extensively validated for western blotting using whole cell lysates.
SOX9 Antibody with Control Lysate
SOX9 antibody recognizes the transcription factor SOX-9, also known as SRY (sex-determining region Y)-box 9 protein or SRY-related HMG-box, gene 9. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2H10
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00211 | WB | 100 µl | ||||
| VMA00211KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human SOX9 antibody recognizes the transcription factor SOX9, also known as SRY (sex-determining region Y)-box 9 protein and SRY-related HMG-box, gene 9.
The protein encoded by the SOX9 gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal (provided by RefSeq, Jul 2008).
Mouse anti Human SOX9 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the SOX9 gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal (provided by RefSeq, Jul 2008).
Mouse anti Human SOX9 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SOX9 detects a band of approximately 70 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 1-205 of human SOX9 (NP_000337) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P48436 Related reagents
- Entrez Gene
- SOX9 Related reagents
- GO Terms
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0005634 nucleus
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of SOX9 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.
Contact Information
Online Payment Methods:
- Invoice / Purchase Order
- Credit Card (Maestro, VISA,MasterCard & VISA Electron)
