SOX9 antibody | OTI2H10

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Anti SOX9 Antibody, clone OTI2H10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti SOX9 Antibody, clone OTI2H10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human SOX9 antibody, clone OTI2H10 (**VMA00211**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 120 second exposure. Arrow points to SOX9 (molecular weight 70 kDa).

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Mouse anti SOX9

Product Type: PrecisionAb Monoclonal
Clone: OTI2H10
Isotype: IgG1
Format: Purified
Specificity: SOX9

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VMA00211 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human SOX9 antibody, clone OTI2H10 recognizes the transcription factor SOX9, also known as SRY (sex-determining region Y)-box 9 protein and SRY-related HMG-box, gene 9.

The protein encoded by the SOX9 gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal (provided by RefSeq, Jul 2008).

Mouse anti Human SOX9 antibody, clone OTI2H10 detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SOX9 detects a band of approximately 70 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-205 of human SOX9 (NP_000337) produced in E. coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.