SOCS-3 antibody | SO1

Mouse anti SOCS-3

Product Type
PrecisionAb Monoclonal
Clone
SO1
Isotype
IgG2b
Format
Purified
Specificity
SOCS-3

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VMA00027
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E WB 100 µl loader
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Mouse anti Human SOCS-3 antibody, clone SO1 recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid member of the suppressor of cytokine signaling family (Starr et al. 1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of janus kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways.

SOCS-3 acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3.

The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, toll-like receptor (TLR) agonists and interferon (IFN) gamma, indicating roles in inflammatory diseases such as arthritis, colitis and Crohn’s disease (Elliott and Johnstone 2004). SOCS-3 expression affects IFN alpha sensitivity in tumors (Brender et al. 2001).

Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization. RBPJ–mediated notch signaling regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).

Mouse anti Human SOCS-3 antibody recognizes human SOCS-3 as a single band of ~25 kDa in MCF7 cell lysates by western blotting under reducing conditions. This antibody also cross reacts with SOCS-3 from murine cell lines.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti SOCS-3 antibody detects a band of approximately 25 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Full length SOCS-3 protein
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for SOCS-3 antibody

  1. Zheng, Y. et al. (2019) Lidocaine Potentiates SOCS3 to Attenuate Inflammation in Microglia and Suppress Neuropathic Pain.
    Cell Mol Neurobiol. 39 (8): 1081-92.

Immunofluorescence

Synonyms
Supressor Of Cytokine Signaling 3
UniProt
O14543
Entrez Gene
SOCS3
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0006916 anti-apoptosis
GO:0007259 JAK-STAT cascade
GO:0040008 regulation of growth
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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