SMC3 antibody | AB02/4E11

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Mouse anti SMC3

Product Type
PrecisionAb Monoclonal
Clone
AB02/4E11
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00610
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human SMC3 antibody recognizes SMC3, also known as BAM, HCAP, CDLS3, CSPG6 or BMH.

The SMC3 protein is a subunit of cohesin, a ubiquitously-expressed complex associated with sister chromatid cohesion, chromatin structure, gene expression, and DNA repair (Hill et al. 2016). Establishment of cohesion between replicated sister chromatids is important for ensuring their correct segregation during anaphase of the cell cycle. Acetylation of SMC3 during S phase is essential for the function of cohesin. Deacetylation of SMC3 during telophase is important for recycling cohesin so it can be reloaded during interphase (Dasgupta et al. 2016). Genes encoding subunits of cohesin are mutated in a range of cancers. Mutations in SMC3 have been found in chronic myelogenous leukemia and glioblastoma (Solomon et al. 2014). A post-translationally modified form of SMC3 is secreted as bamacan (Ghiselli et al. 2003), a basement membrane protein (Ghiselli and Iozzo 2000).

Product Details

Target Species
Human
Western Blotting
Mouse anti SMC3 antibody detects a band of approximately 144 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human SMC3 (aa 903-1217)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9UQE7
Entrez Gene
SMC3
GO Terms
GO:0000236 mitotic prometaphase
GO:0000775 chromosome, centromeric region
GO:0000785 chromatin
GO:0000922 spindle pole
GO:0003777 microtubule motor activity
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0005604 basement membrane
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006281 DNA repair
GO:0007052 mitotic spindle organization
GO:0007062 sister chromatid cohesion
GO:0007091 mitotic metaphase/anaphase transition
GO:0007126 meiosis
GO:0008278 cohesin complex
GO:0009294 DNA mediated transformation
GO:0016363 nuclear matrix
GO:0032876 negative regulation of DNA endoreduplication
GO:0045502 dynein binding
GO:0046982 protein heterodimerization activity
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SMC3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml