SMAD5 antibody | AB01/1F7
Mouse anti SMAD5
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB01/1F7
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- SMAD5
Antibody Quality and Performance Guarantee
| Mouse anti SMAD5 antibody recognizes mothers against decapentaplegic homolog 5, also known as JV5-1 and MADH5. SMADs are a group of transcription factors that transmit signals from TGF-beta at the cell surface to the nucleus (Li et al. 2018). TGF-beta signaling is crucial for adult homeostasis and also proper embryonic development. Related to the function of TGF-beta in development, SMAD5 knockout in mice results in early embryonic lethality. SMAD5 is closely related to SMAD1 and SMAD8, and primarily mediates bone morphogenetic protein (Bmp) signaling (Umans et al. 2007). SMAD5 has been shown to be involved with tumor progression, and SMAD5 appears to be required for chemoresistance in triple negative breast carcinoma cells (Opyrchal et al. 2017). |
|
- Target Species
- Human
- Western Blotting
- Mouse anti SMAD5 detects a band of approximately 55 kDa in K562 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-465 of human SMAD5
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q99717
- Entrez Gene
- SMAD5
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0016021 integral to membrane
- GO:0005829 cytosol
- GO:0030618 transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
- GO:0005667 transcription factor complex
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0009880 embryonic pattern specification
- GO:0016563 transcription activator activity
- View More GO Terms
- GO:0030509 BMP signaling pathway
- GO:0031625 ubiquitin protein ligase binding
VMA00971
100005724
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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