SMAD4 antibody | 1B6

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Anti SMAD4 Antibody, clone 1B6 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SMAD4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 63 second exposure. Arrow points to SMAD4 (molecular weight 65 kDa)

Mouse anti SMAD4

SMAD4 antibody recognizes mothers against decapentaplegic homolog 4, also known as MAD homolog 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4 or deletion target in pancreatic carcinoma 4.

Product Type: PrecisionAb™ Monoclonal
Clone: 1B6
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00252 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human SMAD4 antibody recognizes the Mothers against decapentaplegic homolog 4, also known as MAD homolog 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4 and mothers against decapentaplegic.

SMAD4 encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. A product of SMAD4 gene, Mothers against decapentaplegic homolog 4 forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. (provided by RefSeq, Oct 2009).

Mouse anti Human SMAD4 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti SMAD4 detects a band of approximately 65 kDa in Jurkat cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Purified recombinant fragment of human SMAD4 expressed in E. coli

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q13485 Related reagents
Entrez Gene: SMAD4 Related reagents
GO Terms: GO:0001666 response to hypoxia; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005829 cytosol; GO:0030616 transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; GO:0005813 centrosome; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007183 SMAD protein complex assembly; GO:0010718 positive regulation of epithelial to mesenchymal transition; GO:0010843 promoter binding; GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation; GO:0030308 negative regulation of cell growth; GO:0030509 BMP signaling pathway; GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway; GO:0032444 activin responsive factor complex; GO:0032909 regulation of transforming growth factor-beta2 production; GO:0042177 negative regulation of protein catabolic process; GO:0042803 protein homodimerization activity; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0060021 palate development; GO:0060391 positive regulation of SMAD protein import into nucleus; GO:0060395 SMAD protein signal transduction; GO:0070411 I-SMAD binding; GO:0070412 R-SMAD binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of SMAD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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