SMAD4 antibody | 1B6

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Mouse anti SMAD4

SMAD4 antibody recognizes mothers against decapentaplegic homolog 4, also known as MAD homolog 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4 or deletion target in pancreatic carcinoma 4.

SMAD4 Antibody with Control Lysate

SMAD4 antibody recognizes mothers against decapentaplegic homolog 4, also known as MAD homolog 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4 or deletion target in pancreatic carcinoma 4.

Product Type
PrecisionAb™ Monoclonal
Clone
1B6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00252 WB 100 µl
VMA00252KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SMAD4 antibody recognizes the Mothers against decapentaplegic homolog 4, also known as MAD homolog 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4 and mothers against decapentaplegic.

SMAD4 encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. A product of SMAD4 gene, Mothers against decapentaplegic homolog 4 forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. (provided by RefSeq, Oct 2009).

Mouse anti Human SMAD4 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human SMAD4 expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13485 Related reagents
Entrez Gene
SMAD4 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0030616 transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
GO:0005813 centrosome
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007183 SMAD protein complex assembly
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010843 promoter binding
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030308 negative regulation of cell growth
GO:0030509 BMP signaling pathway
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0032444 activin responsive factor complex
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0042177 negative regulation of protein catabolic process
GO:0042803 protein homodimerization activity
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0060021 palate development
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0060395 SMAD protein signal transduction
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SMAD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SMAD4 detects a band of approximately 65 kDa in Jurkat cell lysates
Western Blotting
Anti SMAD4 detects a band of approximately 65 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB