SAP antibody | AB12-5A1

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Mouse anti Human SAP:Biotin

Product Type
Monoclonal Antibody
Clone
AB12-5A1
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6269B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human SAP antibody, clone AB12-5A1, recognizes Serum Amyloid P-component (SAP) also known as 9.5S alpha-1-glycoprotein, a member of the pentraxin family of proteins. It is synthesized by hepatocytes and secreted into the blood. In humans the levels of SAP range between 20-50 µg/ml (Pilling & Gomer 2018).

SAP is able to bind to a variety of molecules including apoptotic debris, bacterial polysaccharides, amyloid deposits and bacterial toxins. Phagocytic cells such as monocytes and macrophages then bind the SAP and engulf the material bound to SAP as part of debris clearance (Pilling & Gomer 2018).

The biotinylated Mouse anti Human SAP antibody, clone AB12-5A1 (MCA6269B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human SAP antibody, clone AB18-4E2 (MCA6268GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Native SAP purified from human serum
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P02743
Entrez Gene
APCS
GO Terms
GO:0051082 unfolded protein binding
GO:0006457 protein folding
GO:0006953 acute-phase response
GO:0046872 metal ion binding
GO:0005529 sugar binding
GO:0005615 extracellular space
GO:0051131 chaperone-mediated protein complex assembly
Regulatory
For research purposes only

Applications of SAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human SAP MCA6268GA E 0.1 mg loader

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