RXR Alpha antibody | AB02/1D10

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Mouse anti RXR Alpha

Product Type
PrecisionAb Monoclonal
Clone
AB02/1D10
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00797
Datasheet
WB
SDS
100 µl loader

Mouse anti RXR alpha antibody recognizes retinoic acid receptor RXR-alpha, also known as NR2B1.

The retinoid X receptor (RXR) is part of the steroid/thyroid hormone superfamily of nuclear receptors, proteins which predominantly functions as transcription factors with roles in development, cell differentiation, metabolism, and cell death. RXR alpha is one of three known subtypes of RXR. RXR in general, and also RXR alpha are involved with cancer development. For example, a malfunction of RXR alpha is associated with liver cancer (Yamazaki et al. 2007). RXR is upregulated in several cancers, including many ductal breast carcinomas (Rodrigues de Almeida and Conda-Sheridan 2019). Targeting RXR alpha in breast cancer cell lines has been shown to inhibit proliferation, and caused disease regression in animal models (Crowe and Chandraratna 2004).

Product Details

Target Species
Human
Western Blotting
Mouse anti RXR alpha antibody detects a band of approximately 58 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-462 of human RXR alpha
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P19793
Entrez Gene
RXRA
GO Terms
GO:0000790 nuclear chromatin
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003706 ligand-regulated transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0003713 transcription coactivator activity
GO:0004886 retinoid-X receptor activity
GO:0005654 nucleoplasm
GO:0006766 vitamin metabolic process
GO:0008203 cholesterol metabolic process
GO:0008270 zinc ion binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0019899 enzyme binding
GO:0032526 response to retinoic acid
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0042809 vitamin D receptor binding
GO:0044255 cellular lipid metabolic process
GO:0044323 retinoic acid-responsive element binding
GO:0044419 interspecies interaction between organisms
GO:0046982 protein heterodimerization activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RXR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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