RPS6KB1 antibody | CD04/4D3

100% Secure


Mouse anti RPS6KB1

Product Type
PrecisionAb Monoclonal
Clone
CD04/4D3
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00792
Datasheet
WB
SDS
100 µl loader

Mouse anti RPS6KB1 antibody recognizes ribosomal protein S6 kinase beta-1, also known as S6K1 and STK14A.

RPS6KB1 is a ribosomal serine/threonine kinase regulated by the PI3K/mTOR pathway (Chen et al. 2017). This protein has been linked to a range of cellular processes including glucose homeostasis, mRNA processing, protein synthesis, cell growth and survival. RPS6KB1 becomes activated by phosphorylation at a variety of serine or threonine residues. It also may be a driver of tumor initiation and progression, and inactivation of RPS6KB1 has been suggested as a therapy for many cancers (Wang-Bishop et al. 2019). Hyperactivation of RPS6KB1, rather than overexpression, has been found to predict poor prognosis in patients with non-small cell lung cancer (Chen et al. 2017). In prostate cancer cell lines, miR-195 has been found to target RPS6KB1 and has been suggested as a potential therapeutic target (Cai et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti RPS6KB1 antibody detects a band of approximately 73 and 65 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 416-525 of human RPS6KB1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P23443
Entrez Gene
RPS6KB1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004672 protein kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005741 mitochondrial outer membrane
GO:0008286 insulin receptor signaling pathway
GO:0019717 synaptosome
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030054 cell junction
GO:0031929 TOR signaling cascade
GO:0043066 negative regulation of apoptosis
GO:0045202 synapse
GO:0045931 positive regulation of mitotic cell cycle
GO:0045948 positive regulation of translational initiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPS6KB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸


How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra