RPS6KB1 antibody | CD04/4D3
Mouse anti RPS6KB1 antibody recognizes ribosomal protein S6 kinase beta-1, also known as S6K1 and STK14A.
RPS6KB1 is a ribosomal serine/threonine kinase regulated by the PI3K/mTOR pathway (Chen et al. 2017). This protein has been linked to a range of cellular processes including glucose homeostasis, mRNA processing, protein synthesis, cell growth and survival. RPS6KB1 becomes activated by phosphorylation at a variety of serine or threonine residues. It also may be a driver of tumor initiation and progression, and inactivation of RPS6KB1 has been suggested as a therapy for many cancers (Wang-Bishop et al. 2019). Hyperactivation of RPS6KB1, rather than overexpression, has been found to predict poor prognosis in patients with non-small cell lung cancer (Chen et al. 2017). In prostate cancer cell lines, miR-195 has been found to target RPS6KB1 and has been suggested as a potential therapeutic target (Cai et al. 2015).
RPS6KB1 is a ribosomal serine/threonine kinase regulated by the PI3K/mTOR pathway (Chen et al. 2017). This protein has been linked to a range of cellular processes including glucose homeostasis, mRNA processing, protein synthesis, cell growth and survival. RPS6KB1 becomes activated by phosphorylation at a variety of serine or threonine residues. It also may be a driver of tumor initiation and progression, and inactivation of RPS6KB1 has been suggested as a therapy for many cancers (Wang-Bishop et al. 2019). Hyperactivation of RPS6KB1, rather than overexpression, has been found to predict poor prognosis in patients with non-small cell lung cancer (Chen et al. 2017). In prostate cancer cell lines, miR-195 has been found to target RPS6KB1 and has been suggested as a potential therapeutic target (Cai et al. 2015).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti RPS6KB1 antibody detects a band of approximately 73 and 65 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 416-525 of human RPS6KB1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P23443
- Entrez Gene
- RPS6KB1
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0004672 protein kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005741 mitochondrial outer membrane
- GO:0008286 insulin receptor signaling pathway
- GO:0019717 synaptosome
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030054 cell junction
- GO:0031929 TOR signaling cascade
- GO:0043066 negative regulation of apoptosis
- GO:0045202 synapse
- GO:0045931 positive regulation of mitotic cell cycle
- GO:0045948 positive regulation of translational initiation
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RPS6KB1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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