RPS6KB1 antibody | CD04/4D3

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Anti RPS6KB1 Antibody, clone CD04/4D3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RPS6KB1 Antibody, clone CD04/4D3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RPS6KB1 Antibody, clone CD04/4D3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RPS6KB1 antibody (1/2000, **VMA00792**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to RPS6KB1 (molecular weight 73 and 65 kDa)

Anti RPS6KB1 Antibody, clone CD04/4D3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of RPS6KB1 from MCF-7 whole cell lysate. The Input lane is 10% input; the RPS6KB1-IP lane is the immunoprecipitation with the RPS6KB1 antibody (2.5 µg, **VMA00792**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA929). Western blot analysis was performed using RPS6KB1 antibody (1/1000, **VMA00792**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to RPS6KB1.

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WB IP

Mouse anti RPS6KB1

Product Type: PrecisionAb Monoclonal
Clone: CD04/4D3
Isotype: IgG2a
Format: Purified
Specificity: RPS6KB1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00792 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti RPS6KB1 antibody recognizes ribosomal protein S6 kinase beta-1, also known as S6K1 and STK14A.

RPS6KB1 is a ribosomal serine/threonine kinase regulated by the PI3K/mTOR pathway (Chen et al. 2017). This protein has been linked to a range of cellular processes including glucose homeostasis, mRNA processing, protein synthesis, cell growth and survival. RPS6KB1 becomes activated by phosphorylation at a variety of serine or threonine residues. It also may be a driver of tumor initiation and progression, and inactivation of RPS6KB1 has been suggested as a therapy for many cancers (Wang-Bishop et al. 2019). Hyperactivation of RPS6KB1, rather than overexpression, has been found to predict poor prognosis in patients with non-small cell lung cancer (Chen et al. 2017). In prostate cancer cell lines, miR-195 has been found to target RPS6KB1 and has been suggested as a potential therapeutic target (Cai et al. 2015).

Target Species: Human
Western Blotting: Mouse anti RPS6KB1 antibody detects a band of approximately 73 and 65 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 416-525 of human RPS6KB1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P23443
Entrez Gene: RPS6KB1
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0004672 protein kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005741 mitochondrial outer membrane; View More GO Terms; GO:0008286 insulin receptor signaling pathway; GO:0019717 synaptosome; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030054 cell junction; GO:0031929 TOR signaling cascade; GO:0043066 negative regulation of apoptosis; GO:0045202 synapse; GO:0045931 positive regulation of mitotic cell cycle; GO:0045948 positive regulation of translational initiation