RPN1 antibody | OTI5B1

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Anti RPN1 Antibody, clone OTI5B1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RPN1 Antibody, clone OTI5B1 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RPN1 Antibody, clone OTI5B1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human RPN1 antibody, clone OTI5B1 (**VMA00272**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to RPN1 (molecular weight 69 kDa).

Anti RPN1 Antibody, clone OTI5B1 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of RPN1 from K562 whole cell lysate. The Input lane is 10% input; the RPN1-IP lane is the immunoprecipitation with the RPN1 antibody (2.5 µg, **VMA00272**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using RPN1 antibody (1/1,000, **VMA00272**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to RPN1.

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WB IP

Mouse anti RPN1

Product Type: PrecisionAb Monoclonal
Clone: OTI5B1
Isotype: IgG1
Format: Purified
Specificity: RPN1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00272 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human RPN1 antibody, clone OTI5B1 recognizes ribophorin-1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, oligosaccharyltransferase 1 homolog and oligosaccharyltransferase complex subunit (non-catalytic).

Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).

Mouse anti Human RPN1 antibody, clone OTI5B1 detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RPN1 detects a band of approximately 69 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human RPN1 (NP_002941) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P04843
Entrez Gene: RPN1
GO Terms: GO:0005515 protein binding; GO:0016021 integral to membrane; GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity; GO:0008250 oligosaccharyltransferase complex; GO:0018279 protein N-linked glycosylation via asparagine; GO:0019718 rough microsome; GO:0042470 melanosome; GO:0043687 post-translational protein modification