RPN1 antibody | OTI5B1
Mouse anti RPN1
RPN1 antibody recognizes dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, or oligosaccharyltransferase 1 homolog.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI5B1
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00272 | WB | 100 µl |
|
Mouse anti Human RPN1 antibody recognizes ribophorin-1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, oligosaccharyltransferase 1 homolog and oligosaccharyltransferase complex subunit (non-catalytic).
Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).
Mouse anti Human RPN1 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).
Mouse anti Human RPN1 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RPN1 detects a band of approximately 69 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human RPN1 (NP_002941) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04843 Related reagents
- Entrez Gene
- RPN1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity
- GO:0008250 oligosaccharyltransferase complex
- GO:0018279 protein N-linked glycosylation via asparagine
- GO:0019718 rough microsome
- GO:0042470 melanosome
- GO:0043687 post-translational protein modification
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RPN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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