RPN1 antibody | OTI5B1

Mouse anti RPN1

Product Type
PrecisionAb Monoclonal
Clone
OTI5B1
Isotype
IgG1
Format
Purified
Specificity
RPN1

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VMA00272
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IP WB 100 µl loader
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Mouse anti Human RPN1 antibody, clone OTI5B1 recognizes ribophorin-1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, oligosaccharyltransferase 1 homolog and oligosaccharyltransferase complex subunit (non-catalytic).

Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).

Mouse anti Human RPN1 antibody, clone OTI5B1 detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RPN1 detects a band of approximately 69 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human RPN1 (NP_002941) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P04843
Entrez Gene
RPN1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0008250 oligosaccharyltransferase complex
GO:0018279 protein N-linked glycosylation via asparagine
GO:0019718 rough microsome
GO:0042470 melanosome
GO:0043687 post-translational protein modification
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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