RPN1 antibody | OTI5B1
Mouse anti RPN1
RPN1 antibody recognizes dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, or oligosaccharyltransferase 1 homolog.
RPN1 Antibody with Control Lysate
RPN1 antibody recognizes dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, or oligosaccharyltransferase 1 homolog.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI5B1
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00272 | WB | 100 µl | ||||
| VMA00272KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human RPN1 antibody recognizes ribophorin-1, also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, oligosaccharyltransferase 1 homolog and oligosaccharyltransferase complex subunit (non-catalytic).
Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).
Mouse anti Human RPN1 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the RPN1 gene, ribophorin-1encodes is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome (provided by RefSeq, Jul 2008).
Mouse anti Human RPN1 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human RPN1 (NP_002941) produced in HEK293T cells
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04843 Related reagents
- Entrez Gene
- RPN1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity
- GO:0008250 oligosaccharyltransferase complex
- GO:0018279 protein N-linked glycosylation via asparagine
- GO:0019718 rough microsome
- GO:0042470 melanosome
- GO:0043687 post-translational protein modification
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RPN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti RPN1 detects a band of approximately 69 kDa in K562 cell lysates
- Western Blotting
- Anti RPN1 detects a band of approximately 69 kDa in K562 cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
