RAPTOR antibody | 1A9

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Mouse anti RAPTOR

RAPTOR antibody recognizes regulatory-associated protein of mTOR, also known as p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats. This antibody has been extensively validated for western blotting using whole cell lysates.

RAPTOR Antibody with Control Lysate

RAPTOR antibody recognizes regulatory-associated protein of mTOR, also known as p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
1A9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00311 WB 100 µl
VMA00311KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RAPTOR antibody recognizes the regulatory-associated protein of mTOR (RAPTOR), also known as p150 target of rapamycin (TOR)-scaffold protein.

Encoded by the RPTOR gene, regulatory-associated protein of mTOR is a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. RAPTOR forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for RPTOR (provided by RefSeq, Sep 2009).

Mouse anti Human RAPTOR antibody detects a band of 149 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant RAPTOR

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8N122 Related reagents
Entrez Gene
RPTOR Related reagents
GO Terms
GO:0016049 cell growth
GO:0005764 lysosome
GO:0005829 cytosol
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0031669 cellular response to nutrient levels
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0032008 positive regulation of TOR signaling cascade
GO:0032403 protein complex binding
GO:0071230 cellular response to amino acid stimulus
GO:0071902 positive regulation of protein serine/threonine kinase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RAPTOR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti RAPTOR detects a band of approximately 149 kDa in HEK293 cell lysates
Western Blotting
Anti RAPTOR detects a band of approximately 149 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB