RAPTOR antibody | 1A9

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Mouse anti RAPTOR

Product Type
PrecisionAb Monoclonal
Clone
1A9
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00311
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human RAPTOR antibody recognizes the regulatory-associated protein of mTOR (RAPTOR), also known as p150 target of rapamycin (TOR)-scaffold protein.

Encoded by the RPTOR gene, regulatory-associated protein of mTOR is a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. RAPTOR forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for RPTOR (provided by RefSeq, Sep 2009).

Mouse anti Human RAPTOR antibody detects a band of 149 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RAPTOR detects a band of approximately 149 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant RAPTOR

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8N122 Related reagents
Entrez Gene
RPTOR Related reagents
GO Terms
GO:0016049 cell growth
GO:0005764 lysosome
GO:0005829 cytosol
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0031669 cellular response to nutrient levels
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0032008 positive regulation of TOR signaling cascade
GO:0032403 protein complex binding
GO:0071230 cellular response to amino acid stimulus
GO:0071902 positive regulation of protein serine/threonine kinase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RAPTOR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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