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PTPN11/6 antibody | CD01-3E11

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KO Validated
IP-MS Validated
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) thumbnail image 4 Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) thumbnail image 5
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PTPN11/6 antibody (VMA00090) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PTPN11 and PTPN6 (molecular weight 68 kDa).
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates probed with PTPN11/6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PTPN11 and PTPN6 (molecular weight 68 kDa)
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of PTPN11/6 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti PTPN11/6 antibody (VMA00090) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 34 second and (B) 30 second exposure.
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of PTPN11/6 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti PTPN11/6 Antibody, clone CD01-3E11 (PrecisionAb Monoclonal Antibody) gallery image 5
Immunoprecipitation of PTPN11/6 from Jurkat whole cell lysate. The Input lane is 10% input; the PTPN11/6-IP lane is the immunoprecipitation with the PTPN11/6 antibody (2.5 µg, VMA00090); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg,MCA691). Western blot analysis was performed using PTPN11/6 antibody (1/1,000, VMA00090) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to PTPN11/6.

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WB IP

Mouse anti PTPN11/6

Product Type
PrecisionAb Monoclonal
Clone
CD01-3E11
Isotype
IgG2b
Format
Purified
Specificity
PTPN11/6

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Technical Suppport
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VMA00090
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Mouse anti Human PTPN11/6 antibody recognizes the tyrosine-protein phosphatase non-receptor type 11, also known as PTP-2C, protein-tyrosine phosphatase 1D, protein-tyrosine phosphatase 2C and tyrosine-protein phosphatase non-receptor type 11. This antibody has also been shown to recognize the tyrosine-protein phosphatase non-receptor type 6, also known as HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L and SHP1.

The proteins encoded by PTPN11 and the PTPN6 genes are members of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Mouse anti Human PTPN11/6 antibody detects a band of 68 kDa in whole cell lysates. The antibody has been extensively validated for western blotting. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PTPN11/6 detects a band of approximately 68 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli derived recombinant protein of human PTPN11
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q06124
Entrez Gene
PTPN11
GO Terms
GO:0005829 cytosol
GO:0004726 non-membrane spanning protein tyrosine phosphatase activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0034329 cell junction assembly
GO:0050900 leukocyte migration
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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