PTPN1 antibody | OTI1B4

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Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of PTPN1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti PTPN1 antibody (**VMA00185**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 210 second and (B) 30 second exposure.

Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of PTPN1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti PTPN1 Antibody, clone OTI1B4 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates probed with Mouse anti Human PTPN1 antibody, clone OTI1B4 (**VMA00185**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to PTPN1 (molecular weight 50 kDa).

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Mouse anti PTPN1

Product Type: PrecisionAb Monoclonal
Clone: OTI1B4
Isotype: IgG2a
Format: Purified
Specificity: PTPN1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00185 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human PTPN1 antibody, clone OTI1B4 recognizes PTPN1, also known as protein tyrosine phosphatase, placental, protein-tyrosine phosphatase 1B and tyrosine-protein phosphatase non-receptor type 1.

The protein encoded by the PTPN1 gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for PTPN1 (provided by RefSeq, Jul 2013).

Mouse anti Human PTPN1 antibody, clone OTI1B4 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PTPN1 detects a band of approximately 50 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human PTPN1 (NP_002818) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P18031
Entrez Gene: PTPN1
GO Terms: GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0004725 protein tyrosine phosphatase activity; GO:0005789 endoplasmic reticulum membrane; GO:0008270 zinc ion binding; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060337 type I interferon-mediated signaling pathway; View More GO Terms; GO:0060338 regulation of type I interferon-mediated signaling pathway