PTEN antibody | AbD38831eg

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Human anti PTEN

Product Type
PrecisionAb Monoclonal
Clone
AbD38831eg
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00978
Datasheet
WB
SDS
100 µl loader

Human anti PTEN antibody is a recombinant Fab antibody fragment in the format Fab-Max-FH and recognizes phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, also known as phosphatase and tensin homolog (PTEN), MMAC1, and TEP1. The PTEN protein is a phosphatase with activity against protein and phospholipid, and acts as a potent tumor suppressor by antagonizing the PI3K/AKT pathway, whereas overexpression of this pathway drives tumorigenesis (Bermúdez Brito et al. 2015). In this way, PTEN mutations can lead to altered protein synthesis, cell cycle processes, cell growth and DNA repair (Hopkins et al. 2014). Somatic loss-of-function PTEN mutations are therefore common in many human cancers, and PTEN mutations have also been identified in autism spectrum disorder (ASD) (Rodríguez-Escudero et al. 2011). Under normal conditions, PTEN levels are tightly regulated by transciptional, post-transcriptional and post-translational methods, and small changes in PTEN levels can have dramatic effects on cellular function (Haddadi et al. 2018).

Product Details

Target Species
Human
Western Blotting
Human anti PTEN detects a band of approximately 54 kDa in MCF-7 cell lysate
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of an inactive variant of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Protein was generated against the peptide HYRYSDTTDSDP (PTENaa375-388)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P60484
Entrez Gene
PTEN
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000287 magnesium ion binding
GO:0001933 negative regulation of protein phosphorylation
GO:0006915 apoptosis
GO:0004438 phosphatidylinositol-3-phosphatase activity
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0004725 protein tyrosine phosphatase activity
GO:0006470 protein dephosphorylation
GO:0008289 lipid binding
GO:0006917 induction of apoptosis
GO:0007417 central nervous system development
GO:0008138 protein tyrosine/serine/threonine phosphatase activity
GO:0008283 cell proliferation
GO:0009898 internal side of plasma membrane
GO:0010975 regulation of neuron projection development
GO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
GO:0016605 PML body
GO:0050821 protein stabilization
GO:0019899 enzyme binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030165 PDZ domain binding
GO:0030336 negative regulation of cell migration
GO:0046855 inositol phosphate dephosphorylation
GO:0046856 phosphatidylinositol dephosphorylation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051091 positive regulation of transcription factor activity
GO:0051717 inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity
GO:0051800 phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity
GO:0051895 negative regulation of focal adhesion assembly
GO:0051898 negative regulation of protein kinase B signaling cascade
GO:0060070 canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of PTEN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Technical Advice
Technical advice - Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

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