PSMB4 antibody | OTI5B5

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Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human PSMB4 antibody, clone OTI5B5 (**VMA00142**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 20 second exposure. Arrow points to PSMB4 (molecular weight 28 kDa).

Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMB4 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti PSMB4 antibody (**VMA00142**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 300 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.

Anti PSMB4 Antibody, clone OTI5B5 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of PSMB4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMB4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti PSMB4

Product Type: PrecisionAb Monoclonal
Clone: OTI5B5
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00142 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human PSMB4 antibody, clone OTI5B5 recognizes PSMB4, also known as 26 kDa prosomal protein, hsBPROS26, macropain beta chain, multicatalytic endopeptidase complex beta chain, proteasome beta chain, proteasome chain 3, proteasome subunit HsN3 and proteasome subunit beta type-4.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB4 encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit (provided by RefSeq, Jul 2008).

Mouse anti Human PSMB4 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti PSMB4 detects a band of approximately 28 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant protein of human PSMB4 (NP_002787) produced in HEK293T cell
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P28070
Entrez Gene: PSMB4
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0006915 apoptosis; GO:0004298 threonine-type endopeptidase activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005839 proteasome core complex; GO:0006521 regulation of cellular amino acid metabolic process; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0044419 interspecies interaction between organisms; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PSMB4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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