Protein Kinase C Epsilon antibody | CD04/1C7

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Mouse anti Protein Kinase C Epsilon

Protein kinase C epsilon antibody recognizes PKCE, also known as Protein Kinase C Epsilon type.

Product Type
PrecisionAb™ Monoclonal
Clone
CD04/1C7
Isotype
IgG2b
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00636 WB 100 µl
Mouse anti Human protein kinase C epsilon antibody recognizes protein kinase C epsilon type also known PKCE.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCE is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior (provided by RefSeq, Jul 2008).

Mouse anti Human protein kinase C epsilon antibody detects a band of 91 kDa. The antibody has been extensively validated for western blotting using whole cell and tissue lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti protein kinase C epsilon antibody detects a band of approximately 91 kDa in mouse brain lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human protein kinase C epsilon (aa 301-737)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q02156 Related reagents
Entrez Gene
PRKCE Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0007202 activation of phospholipase C activity
GO:0008047 enzyme activator activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Protein Kinase C Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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